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血红蛋白在金-石墨烯修饰电极上的直接电化学
发表时间：2010-09-15 浏览量：2467 下载量：782
| 全部作者： | 何亚萍，郑建斌，李矿田，盛庆林 |
| 作者单位： | 西北大学分析科学研究所 |
| 摘 要： | 构置了基于血红蛋白（hemoglobin, Hb）/离子液体（ionic liquid, IL）/金-石墨烯复合纳米材料（Gold-graphene nano composite material, Au-GE）/碳陶瓷（carbon ceramic electrode, CCE）修饰电极过氧化氢（H2O2）生物传感器,研究了Hb的直接电化学和电催化性质，建立了测定H2O2的伏安新方法。采用拉曼光谱法、紫外光谱法和电化学方法进行了表征。结果表明：血红蛋白在该修饰电极的循环伏安图有一对峰形较好、近似对称的氧化还原峰。H2O2在Hb/IL/Au-GE/CCE修饰电极上有明显的电催化现象，线性范围在2.7×10-7～ 2.5×10-4 mol/L，检测限为1.1×10-7 mol/L（S/N=3），米氏常数KappM为16.6 μmol/L. |
| 关 键 词： | 直接电化学；电催化；过氧化氢电化学传感器；血红蛋白；金-石墨烯复合纳米材料 |
| Title： | Direct electrochemistry of hemoglobin based on Au-GE modified carbon ceramic electrode |
| Author： | HE Yaping, ZHENG Jianbin, LI Kuangtian, SHENG Qinglin |
| Organization： | Institute of Analytical Science, Northwest University |
| Abstract： | A new electrochemical biosensor based on the hemoglobin (Hb)/room temperature ionic liquid (IL)/Gold-graphene nano composite material（Au-GE）/carbon ceramic electrode (CCE) modified electrode was structured for the determination of hydrogen peroxide (H2O2). The direct electrochemistry and electrocatalytic of hemoglobin was studied and a new cyclic voltammetry method for the determination of H2O2 was formed. The resulting Au-GE composite film and the immobilization of Hb was characterized by Raman spectra, UV-vis, and electrochemical methods. Hb/IL/Au-GE/CCE showed a pair of well-defined, quasi-reversible redox peaks. It also showed good electrocatalytic ability to the reduction of H2O2 with the linear 2.7×10-7-2.5×10-4 mol/L and the detection limit was 1.1×10-7 mol/L (S/N=3). The apparent Michaelis-Menten constant (KappM) was estimated to be 16.6 μmol/L. |
| Key words： | direct electrochemistry; electrocatalytic; biosensor of hydrogen peroxide; homoglobin; gold-graphene nano composite material |
| 发表期数： | 2010年9月第17期 |
| 引用格式： | 何亚萍，郑建斌，李矿田，等. 血红蛋白在金-石墨烯修饰电极上的直接电化学[J]. 中国科技论文在线精品论文，2010，3（17）：1742-1746. |
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