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荧光淬灭法测定苏丹红I的方法研究
发表时间：2010-09-15 浏览量：2081 下载量：588
| 全部作者： | 武天丽，王兴民 |
| 作者单位： | 甘肃农业大学理学院 |
| 摘 要： | 采用荧光分光光度计测定苏丹红I，结果表明：色氨酸与苏丹红I结合，从而使色氨酸的荧光淬灭，荧光淬灭程度在一定范围内与苏丹红I的质量浓度在0.001 4～0.009 5 mg/L（R=0.998 4）范围内呈线性关系，据此建立一种色氨酸荧光淬灭法快速测定辣椒及其制品中苏丹红I的含量。方法的检出限为0.000 7 mg/L. 用荧光淬灭法测定苏丹红I的方法新颖、快速、灵敏度高、选择性强、需样量少、简便，用于市售小尖椒和甘谷辣椒及其制品中苏丹红I的测定,结果满意。 |
| 关 键 词： | 无机化学；苏丹红I；荧光淬灭；辣椒；乙腈；色氨酸 |
| Title： | Research on measuration of the sudan I by using fluorenscence quenching Processing method |
| Author： | WU Tianli, WANG Xingmin |
| Organization： | College of Sciences, Gansu Agricultural University |
| Abstract： | After using fluorescence spectrophotometer to measure Sudan I, the reseults show that: when the tryptophan is combined with the Sudan I, the fluorenscence in the tryptophan will be quenched. Within a certain range of 0.001 4-0.009 5 mg/L（R=0.998 4）, the fluorenscence quenching effects has a linear character of relationship with the thickness of Sudan I-acetonitrile, based on this situation a method of determining the Sudan I content quickly in the chili with the adoption of using the tryptophan fluorenscence quenching process has been established. The detection limit of this method is 0.000 7 mg/L, while the method of using the fluorenscence queching process to determine the Sudan I is original, fast, simple, with high delicacy and selectivity, and requires less resource. It is used to determine the Sudan I content in the Tine chili, the Gangu chili and their products, and the results are satisfying. |
| Key words： | inorganic chemistry; Sudan I; fluorescence quenching; chili; acetonitrile; tryptophan |
| 发表期数： | 2010年9月第17期 |
| 引用格式： | 武天丽，王兴民. 荧光淬灭法测定苏丹红I的方法研究[J]. 中国科技论文在线精品论文，2010，3（17）：1757-1762. |
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