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迟缓爱德华氏菌对鱼血浆反应的差异蛋白质组学研究
发表时间:2011-11-15 浏览量:1989 下载量:760
全部作者: | 许文姣,李惠 |
作者单位: | 中山大学生命科学学院,有害生物控制与资源利用国家重点实验室蛋白质组学中心 |
摘 要: | 将同等菌量的迟缓爱德华氏菌(Edwardsiella tarda)与不同体积的鱼血浆孵育,获取全菌蛋白,通过观察等电聚焦双向电泳,发现经过血浆处理后迟缓爱德华氏菌有两个显著上调差异点;进一步质谱鉴定,这两个差异点分别为GTP结合蛋白(YihK)和磷酸甘油酸变位酶(GpmA)。这些发现有助于阐明病原菌对鱼血浆反应的分子机制。 |
关 键 词: | 水产生物学;迟缓爱德华氏菌;蛋白质组学;血浆 |
Title: | Differential proteomic approach for Edwardsiella tarda in response to fish plasma |
Author: | XU Wenjiao, LI Hui |
Organization: | Center for Proteomics, State Key Laboratory of Bio-Control, School of Life Sciences, Sun Yat-Sen University |
Abstract: | In the present study, equal amount bacteria were incubated with different volumes of fish plasma and then bacterial proteins were extracted for 2-DE-based proteomics. Upregulation of YihK and GpmA were detected. The finding is helpful in clarification of molecular mechanism of Edwardsiella tarda in response to fish plasma. |
Key words: | aquaculture biology; Edwardsiella tarda; proteomics; plasma |
发表期数: | 2011年11月第21期 |
引用格式: | 许文姣,李惠. 迟缓爱德华氏菌对鱼血浆反应的差异蛋白质组学研究[J]. 中国科技论文在线精品论文,2011,4(21):2007-2012. |
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