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海洋植物大叶藻EST序列的获得与分析
发表时间：2011-11-15 浏览量：2794 下载量：851
| 全部作者： | 孔凡娜，刘利民，茅云翔 |
| 作者单位： | 中国海洋大学海洋生命学院 |
| 摘 要： | 为了解大叶藻（Zostera marina）的耐盐机制，发掘其耐盐基因，构建大叶藻的全长cDNA文库，通过5′末端测序获得了4 002条高质量的表达序列标签（expressed sequence tags，EST），被组装成241个片段重叠群（contigs）和1 673个克隆子（singletons），代表了1 914个非重复序列（unigenes）。BLASX程序分析显示1 664个unigenes与NCBI nr数据库中的已知基因具有较高的相似性，其中丰度最高的基因为金属硫蛋白（metallothionein）和叶绿素a/b结合蛋白（chlorophyll a/b-binding protein），分别占总EST的7.1%和1.7%. 蛋白相邻类聚簇（clusters of orthologous groups of proteins, COG）分析显示499个基因被注释，KEGG（Kyoto encyclopedia of genes and genomes）分析表明，1 462个基因被分配到161个生化途径中，938个基因得到GO（gene ontology）注释，InterProScan程序分析产生1 003个InterPro家族。相关结果有助于进一步从分子水平理解大叶藻的耐盐机制。 |
| 关 键 词： | 水产生物学；大叶藻；全长cDNA文库；表达序列标签；耐盐基因 |
| Title： | Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species Zostera marina |
| Author： | KONG Fanna, LIU Limin, MAO Yunxiang |
| Organization： | College of Marine Life Sciences, Ocean University of China |
| Abstract： | To investigate salt-tolerant mechanism and discover salt-tolerant genes of Zostera marina, a cDNA library was constructed in which single-pass 5′ sequencing of 4 081 clones yielded 4 002 high-quality expressed sequence tags (ESTs). These ESTs were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. Average length of these ESTs was 582 bp, ranging from 100 bp to 1 500 bp. BLASX analysis revealed that 1 664 unigenes had significant homology to known genes against the NCBI nr database (E value≤10-5). Among them, the two most abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), which accounted for 7.1% and 1.7% of total ESTs, respectively. Compared to the Clusters of orthologous groups (COG) categories, 499 unigenes were annotated (E value≤10-10). Using the Kyoto encyclopedia of genes and genomes (KEGG), 1 462 of the 1914 unigenes were assigned into 161 pathways (E value≤10-5). For gene ontology (GO) hierarchy analysis, a total of 938 unigenes were assigned GO terms. For InterProScan searches, 1 003 InterPro families were recognized. Results of this study will contribute to further understanding of the molecular mechanism of saline tolerance in Z. marina. |
| Key words： | aquaculture biology; Zostera marina; cDNA library; expressed sequence tags; salt-tolerant gene |
| 发表期数： | 2011年11月第21期 |
| 引用格式： | 孔凡娜，刘利民，茅云翔. 海洋植物大叶藻EST序列的获得与分析[J]. 中国科技论文在线精品论文，2011，4（21）：2013-2021. |
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