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gga-miR-92对靶基因ATRX的调控分析
发表时间：2012-06-15 浏览量：1686 下载量：734
| 全部作者： | 王晓，苗楠，龚萍，彭秀丽，龚炎长，俸艳萍 |
| 作者单位： | 华中农业大学农业动物遗传育种与繁殖教育部重点实验室 |
| 摘 要： | 为研究gga- miR- 92在鸡性腺发育中的调控作用，首先通过生物信息学预测与性腺发育相关的靶基因，然后采用双荧光素酶报告基因法在鸡DF- 1细胞中验证miR- 92对靶基因的调控作用。结果表明：TargetScan和miRDB 2个软件预测到X连锁的α地中海贫血/智力障碍综合症（alpha thalassemia/mental retardation syndrome X- linked，ATRX）是gga- miR- 92的靶基因，成功克隆并构建了ATRX 3′UTR的表达质粒，双荧光素酶报告基因分析表明miR- 92能显著抑制融合ATRX 3′UTR报告基因的荧光表达量（P<0.01）。研究结果证明了ATRX与gga- miR- 92间存在负向调控关系。 |
| 关 键 词： | 养禽学；鸡；miR- 92；X连锁的α地中海贫血/智力障碍综合症；性腺发育 |
| Title： | Analysis on regulation of gga-miR-92 on target gene ATRX |
| Author： | WANG Xiao, MIAO Nan, GONG Ping, PENG Xiuli, GONG Yanchang, FENG Yanping |
| Organization： | Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University |
| Abstract： | To study the regulation effect of gga- miR- 92 on chicken gonadal development, targets related to gonadal development were first predicted by using bioinformatics methods, then the regulatory relation of miR- 92 with target was identified by dual luciferase reporter assay in chicken DF- 1 cell. The results showed that alpha thalassemia/mental retardation syndrome X- linked (ATRX) was predicted to be a target of gga- miR- 92, and recombined expression vectors of ATRX 3′- UTR were constructed successfully. Dual luciferase reporter assay showed that miR- 92 decreased the activity of luciferase co- transfected with ATRX vector. It can be concluded that ATRX might be negatively regulated by gga- miR- 92. |
| Key words： | poultry science; chicken; miR- 92; alpha thalassemia/mental retardation syndrome X- linked; gonadal development |
| 发表期数： | 2012年6月第11期 |
| 引用格式： | 王晓，苗楠，龚萍，等. gga-miR-92对靶基因ATRX的调控分析[J]. 中国科技论文在线精品论文，2012，5（11）：1070-1074. |
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