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IL-21在乌鸡免疫器官中的分布及发育变化
发表时间：2012-06-15 浏览量：2158 下载量：537
| 全部作者： | 刘志伟，彭克美，宋卉，刘华珍，陈敏，逯志强，刘银雪 |
| 作者单位： | 华中农业大学动物科学技术学院、动物医学院 |
| 摘 要： | 目的：探讨白细胞介素- 21（interleukin- 21，IL- 21）在乌鸡免疫器官中的分布及发育变化，为乌鸡养殖及疾病防控提供理论依据。方法：采用石蜡切片、免疫组织化学（strept avidin- biotin complex，SABC）技术，对乌鸡免疫器官中IL- 21的分布特点及发育变化进行研究，在观察分析乌鸡免疫器官组织结构特点的基础上，探讨IL- 21在乌鸡生长发育及机体免疫中的作用。结果：1） 在乌鸡胸腺中，IL- 21主要分布在髓质中，皮质中较少。髓质中胸腺小体周围分布有较多的阳性信号，呈棕褐色，阳性细胞多呈圆形，直径大小相近，细胞核不明显。2） 在乌鸡脾脏中，IL- 21主要分布在白髓中，红髓中较少。脾小结周围有较多阳性信号分布，白髓的边缘区和椭圆体附近也有阳性信号分布。阳性细胞近似圆形，细胞直径略比胸腺中阳性细胞小，棕黄色，细胞核不明显。3） 在乌鸡法氏囊中，IL- 21主要分布在黏膜上皮、上皮丛和髓质中，皮质中较少，固有层中也有分布。阳性细胞呈棕黄色，直径差异较大，细胞核不明显。结论：IL- 21是一种细胞因子，在机体免疫中具有重要作用，在乌鸡免疫器官中有大量分布，但不同日龄、不同免疫器官中的含量有一定差异。 |
| 关 键 词： | 家畜组织胚胎学；白细胞介素- 21；乌鸡；免疫器官；分布；发育变化 |
| Title： | The distribution and developmental changing of IL-21 in silkie immune organs |
| Author： | LIU Zhiwei, PENG Kemei, SONG Hui, LIU Huazhen, CHEN Min, LU Zhiqiang, LIU Yinxue |
| Organization： | College of Animal Science and Technology/College of Veterinary Medicine, Huazhong Agricultural University |
| Abstract： | Objective: To investigate interleukin- 21 (IL- 21) distribution characteristics and development changes in silkie immune organs, in order to provide the theoretical basis for breeding and disease control and prevention. Methods: The paraffin section, immunohistochemical techniques were used in the present study. Results: 1) In the thymus of silkie, IL- 21 is mainly distributed in medulla with a small amount in cortex. Around the thymus corpuscle in medulla, there are many positive cells which are brown, round, similar in size and with no obvious nucleus. 2) In silkies' spleen, IL- 21 is mainly distributed in white pulp with a small number in red pulp. More positive cells can be seen around the spleen summary in the white pulp. Positive cells can also be observed near the marginal zone and ellipsoids of the white pulp. The round or nearly round positive cells are brownish- yellow with a smaller diameter than those in the thymus and no obvious nucleus. 3) In silkies' bursa, IL- 21 is mainly distributed in mucosal epithelium, epithelial plexus and medulla with a small amount in cortex and some in lamina propria. The brownish- yellow positive cells differ in diameter and have no clear nucleus. Conclusion: IL- 21 is a kind of cell factor that plays an important role in silkies' immune system and has a wide distribution in the immune organs. The amount of IL- 21 varies in different immune organs and in silkies of different day ages. |
| Key words： | livestock histology embryology; interleukin- 21; silkie; immune organ; distribution; developmental changing |
| 发表期数： | 2012年6月第11期 |
| 引用格式： | 刘志伟，彭克美，宋卉，等. IL-21在乌鸡免疫器官中的分布及发育变化[J]. 中国科技论文在线精品论文，2012，5（11）：1094-1100. |
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