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肌源性IL-6在胰岛素抵抗发生中的作用研究
发表时间：2013-09-15 浏览量：1560 下载量：616
| 全部作者： | 雷雨，王萍，田丽，邢振超，文兵 |
| 作者单位： | 铜仁学院体育系 |
| 摘 要： | 目的：探讨肌源性IL-6在胰岛素抵抗发生中的作用。方法：大鼠正常饮食与高糖高脂饮食8周以形成对照。在此过程中采用8周跑台运动（第1周跑速为17 m/min，时间为50 min，随后每周跑速递增1 m/min，时间递增5 min）和/或注射IL-6 shRNA载体（休息日大鼠尾部静脉注射，每2周1次，共4次）干预，检测空腹胰岛素、空腹血糖及肝、腹脂、骨骼肌IL-6基因表达水平等指标。结果：大鼠高糖高脂饮食8周形成胰岛素抵抗；注射IL-6 shRNA组与对照组相比空腹胰岛素、空腹血糖显著增加(P<0.05，或P<0.01)；注射IL-6 shRNA安静组大鼠肝、腹脂、骨骼肌IL-6基因表达水平显著下降；注射IL-6 shRNA运动组次之；高糖高脂运动组IL-6基因表达水平与安静高糖高脂组IL-6基因表达水平显著升高；运动注射IL-6 shRNA组血糖显著高于运动不注射IL-6 shRNA组(P<0.05，或P<0.01)；8周有氧运动显著降低高糖高脂饮食鼠胰岛素抵抗发生的几率。结论：运动引起的肌源性IL-6具有抑制胰岛素抵抗发生的作用，但并不是唯一因素，且具有组织特异性。 |
| 关 键 词： | 运动人体科学；IL-6；运动；胰岛素抵抗；作用 |
| Title： | Function of muscle-derived IL-6 on insulin resistance |
| Author： | LEI Yu, WANG Ping, TIAN Li, XING Zhenchao, WEN Bing |
| Organization： | Physical Education Department, Tongren University |
| Abstract： | Objective: To study the role of muscle-derived IL-6 in insulin resistance occurence. Methods: The contrast experiments between normal diet and high glucose and fat diet were conducted in rats for 8 weeks. In this process, 8 weeks of treadmill exercise (the running speed was 17 m/min and time was 50 min in the first week, then running speed increased 1 m/min and time increased 5 min progressively every week) and/or injection of IL-6 shRNA vectors (intravenous injection into the rats’ tails on every two Monday, a total of 4 times) were used to intervent. The fasting insulin, fasting blood glucose and liver, abdominal fat, skeletal muscle IL-6 gene expression level and so on were tested. Results: Rats with high glucose and fat diet for 8 weeks have developed insulin resistance. Compared with the control group, fasting insulin and fasting blood glucose of injection of IL-6 shRNA group increased significantly (P<0.05, or P<0.01); liver, abdominal fat, skeletal muscle IL-6 gene expression level of injection of IL-6 shRNA quiet group decreased significantly; injection of IL-6 shRNA exercise group took second place. IL-6 gene expression level of both the high glucose and fat exercise group and the quiet high glucose and fat group was significantly increased; blood glucose of exercise injection of IL-6 shRNA group was significantly higher than that of not injection group (P<0.05, or P<0.01). 8 weeks aerobic exercise could significantly reduce the occurrence rate of insulin resistance in the high glucose and fat diet rats. Conclusion: Muscle-derived IL-6 caused by exercise can inhibit the action of insulin resistance, but not the only factor, which has tissue specificity. |
| Key words： | sports science of human body; IL-6; exercise; insulin resistance; function |
| 发表期数： | 2013年9月第17期 |
| 引用格式： | 雷雨，王萍，田丽，等. 肌源性IL-6在胰岛素抵抗发生中的作用研究[J]. 中国科技论文在线精品论文，2013，6（17）：1684-1690. |
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