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补肝汤调控杏仁核恐惧关键基因Egr-1和GRP表达的研究
发表时间：2013-12-15 浏览量：1661 下载量：484
| 全部作者： | 徐世军，代渊 |
| 作者单位： | 成都中医药大学药学院，四川省中药资源系统研究与开发利用国家重点实验室培育基地；成都中医药大学基础医学院 |
| 摘 要： | 目的：考察补肝经方补肝汤对震后惊恐障碍（post-earthquake panic disorder,PEPD）模型大鼠杏仁核生长反应基因1 (earlygrowth response gene 1，Egr-1)和胃泌素释放肽(astrin-releasing peptide，GRP)基因表达的影响，为补肝汤治疗PEPD提供实验依据。方法：单程长时刺激（single prolonged stress, SPS）法制备创伤后应激障碍(post-traumatic stress disorder，PTSD)大鼠模型，补肝汤干预7 d后，处死大鼠，无菌条件下取杏仁核，采用荧光定量PCR法检测杏仁核中Egr-1和GRP相对表达量。结果：PTSD模型大鼠杏仁核Egr-1和GRP较空白对照组显著高表达（P<0.05）；补肝汤高、中剂量组杏仁核Egr-1和GRP相对表达量均较模型对照组显著降低（P<0.05）。结论：补肝汤能抑制惊恐障碍大鼠杏仁核中Egr-1和GRP的异常高表达，说明补肝汤抗惊恐障碍与抑制杏仁核恐惧关键基因Egr-1和GRP的表达有关。 |
| 关 键 词： | 中药药理学；补肝汤；惊恐障碍；生长反应基因1；胃泌素释放肽基因 |
| Title： | Effect of Bugan decorction on expression of Egr-1 and GRP of the amygdala in rats with post-earthquake panic disorder |
| Author： | XU Shijun, DAI Yuan |
| Organization： | Sichuan Provincial Key Laboratory of Systematic Research and Development of Chinese Medicina Resources, Province-Ministry Co-constructed State Key Lab Incubation Base, College of Pharmacy; College of Basic Medical, Chengdu University of TCM |
| Abstract： | Objective: To analyze the effect of the Bugan decorction on post-earthquake panic disorder (PEPD), and explore the mechanism of this effect on express of early growth response gene 1 (Egr-1) and gastrin-releasing peptide (GRP) in amygdala with panic disorder. Methods: The post-traumatic stress disorder (PTSD) rat model was made by single prolonged stress (SPS), and the rats were killed after the treatment with Bugan decorction for 7 days. Then, the scared central amygdale sample was made in the sterile condition, the real-time PCR was used to determine the expression of the amygdala Egr-1 and GRP in the rats’ amygdaloid nuclear. Results: Compared to the blank control group, the expression of Egr-1 and GRP increased significantly (P<0.05). However, compared with the model group, the treatment of high and medium Bugan decorction decreased the expression of Egr-1 and GRP increased significantly (P<0.05). Conclusion: Bugan decorction can be used to resist the panic disorder by increasing the synthesis of the central amygdaloid brain nuclear Egr-1 and GRP. |
| Key words： | pharmacology of traditional Chinese medicine; Bugan decorction; panic disorder; earlygrowth response gene 1; astrin-releasing peptide gene |
| 发表期数： | 2013年12月第23期 |
| 引用格式： | 徐世军，代渊. 补肝汤调控杏仁核恐惧关键基因Egr-1和GRP表达的研究[J]. 中国科技论文在线精品论文，2013，6（23）：2265-2269. |
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