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板蓝根抗病毒有效部位中的化学成分
发表时间：2013-12-15 浏览量：1524 下载量：698
| 全部作者： | 何立巍，杨婧妍，李祥，李伟 |
| 作者单位： | 南京中医药大学药学院 |
| 摘 要： | 目的：研究板蓝根抗病毒有效部位中化学成分的结构和体外抗病毒活性。方法：板蓝根水提物经大孔树脂吸附，10%，50%（体积比）乙醇洗脱得抗病毒有效部位，经硅胶柱色谱、反相硅胶色谱及Sephadex LH-20反复分离纯化得到单体，测定所得化合物的理化常数和波谱数据，鉴定其化学结构。噻唑蓝（methyl-thiazolyldiphenyl-tetrazolium，MTT）检测所得单体化合物体外对单纯疱疹病毒（herpes simplex virus，HSV）有抑制作用。结果：从板蓝根水提抗病毒有效部位中分离得到10个化合物，分别鉴定为丁香苷（1）、4-（1,2,3-三羟基丙基）-2,6-二甲氧基苯-1-O-β-D-葡萄糖苷（2）、异落叶松脂醇（3）、异落叶松脂醇-4-O-β-D-葡萄糖苷（4）、落叶松脂素-4-O-β-D-葡萄糖苷（5）、落叶松脂素-4，4′-二-O-β-D-二葡萄糖苷（6）、2-羟基-1，4-苯二甲酸（7）、D-甘露醇（8）、吲哚-3-乙腈-6-O-β-D-葡萄糖苷（9）、3-［2′-(5′-羟甲基)呋喃基］-1(2H)-异喹啉酮-7-O-β-D-葡萄糖苷（10）。结论：其中1个化合物（化合物10）为新化合物，4个化合物为首次从板蓝根药材中分离得到。化合物10具有明显的抗HSV-2型病毒作用，药物浓度为0.125 g/L时病毒抑制率为57.31%，半数有效量（50% concentration of inhibition，IC50）为0.093 g/L，治疗指数（therapeutic index，TI）为7.114. |
| 关 键 词： | 中药化学；板蓝根；新化合物；分离；抗病毒 |
| Title： | Chemical composition from antivirus active parts of Radix Isatidis |
| Author： | HE Liwei, YANG Jingyan, LI Xiang, LI Wei |
| Organization： | College of Pharmacy, Nanjing University of Chinese Medicine |
| Abstract： | Objective: To research the the structure and chemical composition of antiviral effective activity part of Radix Isatidis. Methods: The aqueous extract of Radix Isatidis was absorbed by macroporous resin, the effective antiviral parts were obtained through 10%, 50% ethanol, and the purified monomer was obtained by silica gel column chromatography, reversed phase chromatography on silica gel and Sephadex LH-20 repeatedly separation. Then their physico-chemical constants and spectral data were evaluated and their chemical structures were identified. The inhibitory effect on herpes simplex virus (HSV) of resulting compounds were detected by methyl-thiazolyldiphenyl-tetrazolium (MTT) assay in vitro. Results: Ten compounds were isolated from the active part of water extract, they were respectively identified as syringin (1), 4-(1,2,3-trihydroxypropyl)-2,6-dimethoxyphenyl-1-O-β-D-glucopyranoside (2), isolariciresino (3), isolariciresinol-4-O-β-D-glucopyranoside (4), lariciresinol-4-O-β-D-glucopyranoside (5), lariciresinol-4,4′-bis-O-β-D-glucopyranoside (6), 2-hydroxy-1,4-benzenedi carboxylic acid (7), D-mannitol (8), Indole-3-acetonitrile6-O-β-D-glucopyranoside (9), 3-［2′-(5′-hydroxymethyl)furyl］-1(2H)-isoquinoline-7-O-β-D- glucopyranoside (10). Conclusion: One of these compounds was a new compound, and four were isolated from Radix Isatidis at the first time. The new compound 10 had obvious effects against HSV-2. When the drug concentration was 0.125 g/L, the viral suppression was 57.31%, 50% concentration of inhibition (IC50) was 0.093 g/L, and therapeutic index (TI) was 7.114. |
| Key words： | traditional Chinese medicine chemistry; Radix Isatidis; new compound; isolation; antivirus |
| 发表期数： | 2013年12月第23期 |
| 引用格式： | 何立巍，杨婧妍，李祥，等. 板蓝根抗病毒有效部位中的化学成分[J]. 中国科技论文在线精品论文，2013，6（23）：2275-2282. |
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