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珍珠透骨草降血糖活性部位筛选研究
发表时间：2013-12-15 浏览量：1530 下载量：658
| 全部作者： | 张林林，张辉，孙佳明 |
| 作者单位： | 长春中医药大学中医药与生物工程研发中心 |
| 摘 要： | 目的：筛选珍珠透骨草降血糖有效部位。方法：珍珠透骨草乙醇提取物经氯仿、乙酸乙酯、正丁醇等不同极性溶剂进行萃取得到各部位，采用α-葡萄糖苷酶及α-淀粉酶体外抑制活性模型，测试珍珠透骨草各萃取部位对α-葡萄糖苷酶及α-淀粉酶的抑制活性，筛选并确定珍珠透骨草降血糖的有效部位。结果：珍珠透骨草经氯仿萃取部位α-葡萄糖苷酶抑制作用明显强于其他极性部位，且接近于阿卡波糖，而各萃取部位α-淀粉酶抑制作用明显弱于阿卡波糖。结论：氯仿萃取部分为珍珠透骨草降血糖的有效部位。 |
| 关 键 词： | 中药化学；透骨草；α-葡萄糖苷酶；α-淀粉酶；体外抑制；有效部位 |
| Title： | Screening study on hypoglycemic active site of pearl Speranskia tuberculata |
| Author： | ZHANG Linlin, ZHANG Hui, SUN Jiaming |
| Organization： | Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine |
| Abstract： | Objective: Screening the hypoglycemic effective parts of Speranskia tuberculata. Methods: The ethanol extract of S.tuberculata was extracted successively by chloroform, ethyl acetate and n-butanol, and the different extraction sites were obtained. Then, we used α-glucosidase enzyme and α-amylase inhibitory activity model in vitro to test the inhibitory activity of each extraction site of S.tuberculata on α-glucosidase enzyme and α-amylase, and screened and determined the hypoglycemic effective parts of S.tuberculata. Results: The α-glucosidase inhibitory effect of S.tuberculata chloroform parts was obviously stronger than other polar parts, and its inhibitory effect was closed to the acarbose. While the α-amylase inhibition effect of every extraction site was significantly weaker than that of acarbose. Conclusion: The chloroform extraction part is the hypoglycemic effective parts of the S.tuberculata. |
| Key words： | traditional Chinese medicine chemistry; Speranskia tuberculata; α-glucosidase; α-amylase; inhibition in vitro; effective fraction |
| 发表期数： | 2013年12月第23期 |
| 引用格式： | 张林林，张辉，孙佳明. 珍珠透骨草降血糖活性部位筛选研究[J]. 中国科技论文在线精品论文，2013，6（23）：2283-2286. |
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