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骨髓间充质干细胞共培养对大鼠肝癌细胞增殖的影响
发表时间：2014-09-15 浏览量：1853 下载量：748
| 全部作者： | 郭庆，李晓明，宋关斌 |
| 作者单位： | 昆明学院生命科学与技术系；重庆大学生物工程学院 |
| 摘 要： | 目的：探讨肝癌细胞在间充质干细胞（mesenchymal stem cells，MSCs）共存条件下增殖行为的变化特点及相关分子机理。方法：原代分离培养大鼠骨髓MSCs，采用transwell系统进行MSCs和大鼠肝癌细胞CBRH-7919共培养，用MTT方法分析共培养条件下CBRH-7919细胞的增殖情况，用RT-PCR和Western blotting技术检测相关分子mRNA和蛋白水平的表达变化。结果：与MSCs共培养明显促进CBRH-7919细胞的增殖能力，并诱导CBRH-7919细胞Wnt5a, β-catenin基因的表达和β-catenin蛋白水平的表达。结论：MSCs分泌相关因子可能通过Wnt5a, β-catenin信号促进CBRH-7919细胞的增殖。 |
| 关 键 词： | 细胞生物学；间充质干细胞；肝癌细胞；共培养；增殖；分子机理 |
| Title： | Effect of cocultured bone marrow mesenchymal stem cells on the proliferation of rat hepatoma cells |
| Author： | GUO Qing, LI Xiaoming, SONG Guanbin |
| Organization： | Department of Life Science and Technology, Kunming University; College of Bioengineering, Chongqing University |
| Abstract： | Objective: To study the change of proliferation and correlated molecular mechanism of hepatoma cells under the condition of co-culture with mesenchymal stem cells (MSCs). Methods: using a transwell system, we co-cultured rat hepatoma cells (CBRH-7919) with rat bone marrow-derived MSCs, and then examined the effect of MSCs on proliferation of CBRH-7919 cells. MTT assay was used to check the proliferation, RT-PCR and Western blotting technology were used to check the mRNA and protein changes. Results: The co-culture with MSCs and showed that a significant increase of proliferation of CBRH-7919 cells, and induced the expression of Wnt5a, β-catening enes and β-catenin protein. Conclusion: MSCs could The results demonstrated that MSCs could promote CBRH-7919 cells proliferation, through Wnt5a/β-catenin pathways. |
| Key words： | cell biology; mesenchymal stem cells; hepatoma cells; co-culture; proliferation; molecular mechanism |
| 发表期数： | 2014年9月第17期 |
| 引用格式： | 郭庆，李晓明，宋关斌. 骨髓间充质干细胞共培养对大鼠肝癌细胞增殖的影响[J]. 中国科技论文在线精品论文，2014，7（17）：1716-1722. |
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