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活化-冻干壳聚糖微球固定脂肪酶的研究
发表时间：2014-09-15 浏览量：1761 下载量：488
| 全部作者： | 刘君腾，赵晨希，陈旸，任钟旗，张卫东 |
| 作者单位： | 北京化工大学化学工程学院 |
| 摘 要： | 研究采用真空冷冻干燥技术制备了具有空间网状结构的壳聚糖微球，考察了冻干对载体形貌及活性位点的影响。以活化-冻干处理后的壳聚糖微球为载体固定假丝酵母脂肪酶(Lipase EC3.1.1.3)，对固定化效果进行研究，并与直接固定和活化-固定的固定化酶进行对比，考察了活化过程对固定化酶性能的影响。结果表明，采用活化-冻干-固定法制备的固定化酶性能优良，其固定脂肪酶的水解活力可达57.2 IU/g. |
| 关 键 词： | 生物化学；固定化；活化-冻干；脂肪酶；壳聚糖 |
| Title： | Study on lipase immobilization of activated-freeze-drying chitosan microsphere |
| Author： | LIU Junteng, ZHAO Chenxi, CHEN Yang, REN Zhongqi, ZHANG Weidong |
| Organization： | College of Chemical Engineering, Beijing University of Chemical Technology |
| Abstract： | In this paper, vacuum freeze-drying technology was used to prepare the chitosan microspheres, which had three-dimensional spatial network structure. The effects of vacuum freeze-drying on the morphology of microspheres and active sites were investigated. Candida lipase (Lipase EC3.1.1.3) were immobilized on the activated-lyophilized chitosan microspheres. The immobilization performance of these microspheres was studied and compared with direct-immobilization and activated-immobilization. The influence of activation process on the performance of immobilized lipase was also illustrated. The experimental results indicated that using activated-lyophilized chitosan microspheres as carrier to immobilize lipase is an optimal choice, since the activity of immobilized lipase was over 57.2 IU/g. |
| Key words： | biochemistry; immobilization; activated-freeze-drying; lipase; chitosan |
| 发表期数： | 2014年9月第17期 |
| 引用格式： | 刘君腾，赵晨希，陈旸，等. 活化-冻干壳聚糖微球固定脂肪酶的研究[J]. 中国科技论文在线精品论文，2014，7（17）：1742-1746. |
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