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中肋骨条藻计数及死/活分析的荧光染色法研究
发表时间：2015-02-15 浏览量：1755 下载量：505
| 全部作者： | 孟凡鹏，陶平 |
| 作者单位： | 大连海事大学环境科学与工程学院 |
| 摘 要： | 以典型赤潮藻中肋骨条藻（Skelrtonema costatum）为研究对象，采用荧光染色法计数，建立双醋酸荧光素（fluorescein diacetate，FDA）荧光染色判断中肋骨条藻细胞死/活及计数的方法。结果表明：当FDA浓度为100.0 mg/L、染色时间为10 min时，对浓度为1.0×106 cells/mL的中肋骨条藻染色率为100%；采用所建立方法对含死细胞0，25%，50%，75%，100%的中肋骨条藻细胞液进行染色计数，测定值与配比值基本吻合，拟合曲线的线性相关值R2达0.997. |
| 关 键 词： | 生物无机化学；双醋酸荧光素；中肋骨条藻；荧光染色；计数；死/活分析 |
| Title： | Study on fluorescent staining of counting Skeletonema costatum and its dead/alive assay |
| Author： | MENG Fanpeng, TAO Ping |
| Organization： | College of Environmental Science and Engineering, Dalian Maritime University |
| Abstract： | In this paper, a typical red tide algas, Skelrtonema costatum, was studied. Counting by fluorescent staining, a method to determine and count the dead/alive S. costatum cells was established by using fluorescein diacetate (FDA). The results showed that the dyeing rate of S. costatum (1.0�06 cells/mL) was 100%, when the concentration of FDA was 100.0 mg/L and the dyeing time was 10 min. Using the established method by counting the algae solution of S. costatum containing 0, 25%, 50%, 75%, 100% of dead cells, we found that the measured value was basically equal to the distribution value and the R2 value of the fitting curve was 0.997. |
| Key words： | bioinorganic chemistry; fluorescein diacetate; Skelrtonema costatum; fluoresence staining; counting; dead/live assay |
| 发表期数： | 2015年2月第3期 |
| 引用格式： | 孟凡鹏，陶平 . 中肋骨条藻计数及死/活分析的荧光染色法研究 [J]. 中国科技论文在线精品论文，2015，8（3）：246-250. |
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