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JNK信号转导通路在AngⅡ诱导的血管内皮细胞衰老中的作用机制
发表时间：2015-03-15 浏览量：2129 下载量：885
| 全部作者： | 单海燕，韦晓洁，于凯，赵心，于晓松 |
| 作者单位： | 中国医科大学附属第一医院全科医学科 |
| 摘 要： | 目的：探讨血管紧张素Ⅱ（angiotensinⅡ，AngⅡ）对人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）衰老的影响，以及c-Jun氨基末端激酶（c-Jun-NH2-terminalkinase，JNK）、核转录因子-κB （nuclear factor-κB，NF-κB）表达的变化。方法：制备RPMI1640培养液（含10-6 mol/L AngⅡ）培养HUVECs，通过β-半乳糖苷酶（β-galactosidase，β-gal）染色、流式细胞仪检测细胞衰老；Western-blotting测定JNK，磷酸化JNK（p-JNK），NF-κB的蛋白水平。结果：AngⅡ诱导组β-gal阳性细胞数与对照组相比增长了（81.09±6.72）%；流式细胞仪检测细胞周期停滞于G0-G1期；与对照组相比，AngⅡ诱导16，32，48 h，p-JNK蛋白表达水平显著增高（P<0.05），p-JNK水平于32 h达到最高峰（P<0.01）；p-JNK表达呈持续增加时，NF-κB呈显著上升趋势。结论：AngⅡ诱导内皮细胞的衰老可能与JNK/NF-κB信号转导通路有关。 |
| 关 键 词： | 心血管病学；血管紧张素Ⅱ；内皮细胞；血管衰老；c-Jun氨基末端激酶；核转录因子-κB |
| Title： | Effect of JNK signaling transduction pathway in vascular endothelial cell senescence induced by AngⅡ |
| Author： | SHAN Haiyan, WEI Xiaojie ,YU Kai, ZHAO Xin, YU Xiaosong |
| Organization： | Department of General Practice, The First Hospital of China Medical University |
| Abstract： | Objective: To explore the effect of angiotensinⅡ(AngⅡ) on senescence of human umbilical vein endothelial cells (HUVECs) and the expression change of c-Jun-NH2-terminalkinase (JNK) and nuclear factor-κB (NF-κB). Methods: HUVECs were cultured in RPMI1640 medium including AngⅡ (10-6 mol/L). β-galactosidase (β-gal) staining and flow cytometry were used to identify cell aging status. By means of Western-blotting, the expression of JNK, p-JNK and NF-κB was observed. Results: The number of positive cells which were identified by β-gal staining was significantly higher in AngⅡ-induced cells (81.09±6.72)% than those in the control cells. Flow cytometry showed that the cell cycle was at G0-G1 stage. Compared with control group, p-JNK, NF-κB protein expression increased markedly (P<0.05) induced by AngⅡ for 16, 32, 48 h, and the expression level of p-JNK reached the peak at 32 h (P<0.01). Furthermore, when the expression of p-JNK increased, the NF-κB expression also increased significantly. Conclusion: There is a probability that activated JNK/NF-κB signaling transduction pathway is involved in the process of endothelial cell senescence induced by AngⅡ. |
| Key words： | cardiovascular diseases；angiotensinⅡ; endothelial cell; vascular aging; c-Jun-NH2- terminalkinase; nuclear factor-κB |
| 发表期数： | 2015年3月第5期 |
| 引用格式： | 单海燕，韦晓洁，于凯，等. JNK信号转导通路在AngⅡ诱导的血管内皮细胞衰老中的作用机制[J]. 中国科技论文在线精品论文，2015，8（5）：443-448. |
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