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碳酸酐酶微生物诱导矿化修复混凝土裂缝可行性探究
发表时间：2015-05-31 浏览量：1913 下载量：534
| 全部作者： | 任立夫，钱春香 |
| 作者单位： | 东南大学材料科学与工程学院，绿色建材技术研究所 |
| 摘 要： | 研究选用一类嗜碱芽孢杆菌，其代谢产物碳酸酐酶（carbonic anhydrase，CA）可促进CO2可逆水合反应。实验证明，在提供充足Ca2+条件下，此菌具有修复混凝土裂缝的能力。通过对纯化菌株的生长曲线测定，确定其最佳培养时间为24～28 h，最适pH值为8，随着pH值升高，CA菌生长能力和酶化活力下降，但pH值为12～13时仍存在活菌，并且能够维持一定酶化活力，其对混凝土环境具有一定的耐受性。在溶液环境中，证明了CA菌具有加速CaCO3沉积的能力。进一步在混凝土裂缝自修复试验中，验证了提供充足Ca2+条件下，CA微生物诱导矿化修复混凝土裂缝具有可行性。 |
| 关 键 词： | 建筑材料；材料学；碳酸酐酶；混凝土；裂缝；修复 |
| Title： | Feasibility study on restoration of concrete cracks by carbonic anhydrase microbial-induced mineralization |
| Author： | REN Lifu, QIAN Chunxiang |
| Organization： | School of Materials Science and Engineering, Research Institute of Green Construction Materials, Southeast University |
| Abstract： | A class of alkalophilic Bacillus was used. It can produce carbonic anhydrase (CA) which can promote reversible hydration reaction of CO2. The research shows that the bacteria has the ability to repair concrete cracks provided for abundent Ca2+. Test of growth curve by cultivation experiments determines that the optimal incubation time is 24-28 h and the optimal pH value is 8. With the pH value increasing, the growth capacity of bacteria and the activity of CA are both decreased. There are still viable bacteria which can maintain a certain enzymatic activity when the pH value increases to 12-13, which proves that the alkalophilic Bacillus is compatible with concrete. The bacteria has the ability to accelerate the deposition of CaCO3 in the solution. Further tests verify the bacteria has the ability to repair concrete cracks by CA microbiologically precipitation CaCO3 when providing adequate Ca2+ in concrete. |
| Key words： | building materials; materials science; carbonic anhydrase; concrete; cracks; repair |
| 发表期数： | 2015年5月第10期 |
| 引用格式： | 任立夫，钱春香. 碳酸酐酶微生物诱导矿化修复混凝土裂缝可行性探究[J]. 中国科技论文在线精品论文，2015，8（10）：1022-1031. |
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