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5-HT1D受体激活对小鼠小直径三叉神经节神经元A型K+电流的影响
发表时间：2015-06-15 浏览量：2982 下载量：1049
| 全部作者： | 张怡，陶金 |
| 作者单位： | 苏州大学医学部基础医学与生物科学学院 |
| 摘 要： | 目的：研究5-HT1D受体的激活对小鼠小直径三叉神经节（trigeminal ganglion，TG）神经元A型钾离子电流的影响。方法：培养小鼠TG神经元细胞，利用全细胞膜片钳技术研究5-HT1D受体的激活对小直径TG神经元A型钾电流作用，并通过药理学方法进一步研究其信号通路作用机制。结果：1）电生理研究表明，治疗偏头痛的药物舒马曲坦（sumatriptan）对小直径TG神经元细胞上的A型钾离子电流具有增强作用；2）5-HT1D受体的激活对小直径TG神经元细胞上的A型钾离子电流的增强作用具有剂量依赖性。10 nmol "L"1 L694247（5-HT1D受体的激动剂）对A型钾电流的增长率约为67.8%，5-HT1B受体的激活对小直径TG神经元细胞上的A型钾离子电流不具有增强作用；3）在信号通路方面，蛋白激酶C（PKC）的选择性阻断剂GF109203X白屈菜赤碱（chelerythrine chloride）无法阻断L694247对A型钾电流的增强作用。结论：L694247可通过作用于小鼠小直径TG神经元上的5-HT1D受体来增强A型钾电流；PKC信号通路不参与5-HT1D受体对A型钾电流的增强作用。 |
| 关 键 词： | 基础医学其他学科；5-HT1D受体；A型钾电流；三叉神经节神经元；偏头痛；舒马曲坦 |
| Title： | Effects of A-type K+ currents by the 5-HT1D receptor activation involving migraine in small trigeminal ganglion neurons in mice |
| Author： | ZHANG Yi, TAO Jin |
| Organization： | School of Biology & Basic Medical Sciences, Medical College of Soochow University |
| Abstract： | Objective: To investigate the activation of 5-HT1D receptors affecting the A-type K+ currents in trigeminal ganglion (TG) neurons and its possible mechanisms. Methods: By cultivating TG neurons in mice, the whole-cell patch clamp recording was used to investigate the effect of 5-HT1D receptor on A-type K+ currents in minor diameter TG neurons. Pharmacological methods were used to further explore its signaling pathways. Results: 1) Electrophysiology results reveal that sumatriptan used for treating migraine increases A-type K+ currents in mice’s minor diameter TG neurons; 2) The activation of 5-HT1D receptors increases A-type K+ currents in a dose-dependent manner in mice’s minor diameter TG neurons. 10 nmol"L"1 L694247 (agonist of 5-HT1D receptor) increases A-type K+ currents by about 67.8%. The activation of 5-HT1B receptors doesn’t increase A-type K+ currents in mice’s minor diameter TG neurons; 3) In signal pathway, GF109203X (chelerythrine chloride), the selective blocker of protein kinase C (PKC) pathway, was not involved in the 5-HT1D receptor-mediated effect. Conclusion: L694247 can activate 5-HT1D receptors in minor-diameter TG neurons to increase A-type K+ currents, while PKC pathway is not involved in the 5-HT1D receptor-mediated effect. |
| Key words： | other subjects of basic medicine; 5-HT1D receptors; A-type K+ currents; trigeminal ganglion neurons; migraine; sumatriptan |
| 发表期数： | 2015年6月第11期 |
| 引用格式： | 张怡，陶金. 5-HT1D受体激活对小鼠小直径三叉神经节神经元A型K+电流的影响[J]. 中国科技论文在线精品论文，2015，8（11）：1173-1180. |
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