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外源水杨酸对葡萄土壤微生物群落功能多样性及代谢活性的影响

发表时间:2015-11-15  浏览量:2059  下载量:760
全部作者: 李坤,王博,谢洪刚,郭修武
作者单位: 沈阳农业大学园艺学院;辽宁经济管理干部学院财政金融系
摘 要: 为探讨水杨酸对葡萄微生物群落的调控机制,采用盆栽避雨栽培方式,以贝达葡萄(Vitis riparia×V. labrusca Beta)扦插苗为试材,将外源水杨酸按不同浓度(0.25,0.50,1.00,1.50,2.00 mg/g)施入无葡萄(组1)和有葡萄(组2)栽培的盆栽土壤中,采用Biolog-ECO技术测定土壤中微生物群落功能多样性及代谢活性的变化,同时采用氨基酸自动分析仪检测葡萄根系分泌物中氨基酸的含量。结果表明,外源水杨酸改变了2种土壤中微生物群落功能多样性及代谢活性,且种植葡萄的土壤中微生物代谢活性和群落功能多样性(1.50 mg/g处理除外)高于未种植葡萄的土壤,表现出很强的根际效应。氨基酸测定结果表明,随处理时间延长,水杨酸能提高葡萄分泌氨基酸能力。同时发现,外源水杨酸对2种土壤中微生物代谢活性的影响不同,低浓度水杨酸处理(0.25,0.50 mg/g)在未种植葡萄的土壤中,能够增强微生物代谢活性,但在种植葡萄的土壤中,反而降低了微生物的代谢活性。这说明水杨酸可能与根系分泌物发生了某种互作,进而影响微生物的代谢活性。
关 键 词: 果树学;微生物代谢活性;Biolog-ECO技术;葡萄;自毒物质
Title: Effect of exogenous salicylic acid on microbial community function diversity and metabolism activity of grape planting soil
Author: LI Kun, WANG Bo, XIE Honggang, GUO Xiuwu
Organization: College of Horticulture, Shenyang Agricultural University; Department of Finance, Liaoning Economic Management Cadre College
Abstract: In order to study the regulatory mechanism of salicylic acid on microbial community, we used rhizosphere soil of pot culture with Beta (Vitis riparia譜. labrusca Beta) grapevine cuttings planted on (Group 1) and bulk soil without grapevine cuttings (Group 2) as research subjects under rain-shelter condition. Different concentrations salicylic acids (0.25, 0.50, 1.00, 1.50, 2.00 mg/g) were applied into the soils of Group 1 and Group 2. Soil microbial function diversities and metabolism activities in two kinds of soil were assessed by Biolog-ECO microplate, and amino acid concentrations in root exudates of Beta cuttings were determined by automatic amino acid analyzer. The results showed that salicylic acid could change the microbial function diversities and metabolism activities of two soils, and the function diversity and metabolism activity in soil (except for 1.50 mg/g treatment) with grapevine cuttings planted on (Group 1) were higher than that in soil without grapevine cuttings (Group 2), which showed a strong rhizosphere effect. Meanwhile, the result of amino acid determination showed that salicylic acid could increase the amino acid content in grape root exudates with the prolongation of treating time. There were existing differences on soil microbial metabolism activities between Group 1 and Group 2. Low concentration of salicylic acid (0.25, 0.50 mg/g) could enhance soil microbial metabolism activity in Group 1, however decreased it in Group 2. The results indicated there might be some interaction between salicylic acid and root exudates, which further influenced the microbial metabolism activity.
Key words: pomology; microbial metabolism activity; Biolog-ECO technique; grape; autotoxin
发表期数: 2015年11月第21期
引用格式: 李坤,王博,谢洪刚,等. 外源水杨酸对葡萄土壤微生物群落功能多样性及代谢活性的影响[J]. 中国科技论文在线精品论文,2015,8(21):2241-2246.
 
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