您的位置:首页  > 论文页面

不同马铃薯品种中抗晚疫病基因的分布

发表时间:2015-11-15  浏览量:1972  下载量:524
全部作者: 关雅静,张岩,程永芳,石磊
作者单位: 宁夏农林科学院农业生物技术研究中心;宁夏大学生命科学学院
摘 要: 为理清目前宁夏地区马铃薯栽培和育种中应用较广的20个马铃薯品种中是否存在晚疫病抗性相关基因,针对StR1,StR3a,StRpi,StR1A3,StRB,StRPM1,StRIN4,StPK1等晚疫病抗性基因的序列设计了特异性引物,应用RT-PCR,PCR方法以及QIAxcel全自动DNA/RNA分析系统对每个马铃薯品种进行了检测,确定了这些抗性基因在不同马铃薯品种中的分布情况。结果显示,具有较多抗性基因的马铃薯品种普遍具有较强的晚疫病抗性。通过序列比对分析确定不同马铃薯品种中晚疫病抗性基因均高度保守。研究为马铃薯抗晚疫病育种工作和育种材料晚疫病抗性筛选鉴定提供了分子依据。
关 键 词: 抗病虫害育种;马铃薯;晚疫病;抗性基因
Title: Distribution of late blight resistance genes in different potato cultivars
Author: GUAN Yajing, ZHANG Yan, CHENG Yongfang, SHI Lei
Organization: Agricultural Biotechnology Research Center, Ningxia Academy of Agriculture and Forestry Sciences; School of Life Science, Ningxia University
Abstract: In order to find these late blight resistant genes in 20 potato cultivars that used for cultivation and breeding in Ningxia, we designed specific primers for resistance gene such as StR1, StR3a, StRpi, StR1A3, StRB, StRPM1, StRIN4, StPK1, used RT-PCR, PCR methods and QIAxcel automatic DNA/RNA system, to determine these potato cultivars. We clarified the distribution of these resistance genes in different potato cultivars in this paper. The results indicate that the potato cultivars with more resistance gene are more survivable than the rest of potato cultivars, and sequence alignment analysis shows that these resistance genes are all very conserved. This work provides a molecular basis for late blight resistant potato breeding and screening.
Key words: breeding for diseases and pests resistance; potato; late blight disease; resistance gene
发表期数: 2015年11月第21期
引用格式: 关雅静,张岩,程永芳,等. 不同马铃薯品种中抗晚疫病基因的分布[J]. 中国科技论文在线精品论文,2015,8(21):2251-2257.
 
0 评论数 0
暂无评论
友情链接