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培养的肥厚心肌细胞中RhoA/ROCK通路和ERK通路的相互对话
发表时间：2016-03-15 浏览量：1743 下载量：542
| 全部作者： | 李乐，张可，陶厚权，尚好，张彩玲 |
| 作者单位： | 浙江工业大学药学院；浙江省人民医院中心实验室；浙江人才学院 |
| 摘 要： | 目的：探讨心肌肥厚（cardiac hypertrophy，CH）心肌细胞中RhoA（ras homologue A）/ROCK（Rho相关激酶）通路和细胞外信号调节蛋白激酶（extra cellular signal regulated protein kinase，ERK）通路是否存在相互作用。方法：原代培养的大鼠乳鼠心肌细胞，分为四组：对照组、RhoA组、ERK阻断剂（PD98059）组和RhoA+ERK阻断剂（PD98059）组。分别采用Western blotting法检测CH心肌细胞中ROCK、ERK1/2蛋白表达及磷酸化ROCK（p-ROCK）、磷酸化ERK1/2（p-ERK1/2）蛋白含量，逆转录PCR（reverse transcription PCR，RT-PCR）方法测CH心肌细胞中ROCK蛋白的表达。结果：与对照组相比，RhoA组p-ROCK、p-ERK1/2蛋白含量增多（P<0.05），ERK阻断剂组p-ROCK蛋白含量无差异、p-ERK1/2蛋白含量减少（P<0.05），RhoA+ERK阻断剂组p-ROCK、p-ERK1/2蛋白含量无差异；与RhoA组相比，RhoA+ERK阻断剂组p-ROCK、p-ERK1/2蛋白含量减少（P<0.05）。各组间ROCK、ERK1/2蛋白表达无差异。各组的ROCK mRNA表达差异显著。结论：RhoA可直接活化ROCK蛋白和ERK1/2通路，ERK1/2通路可能位于RhoA/ROCK通路的下游，ROCK蛋白的活化作用可能部分依赖于ERK1/2通路的激活。 |
| 关 键 词： | 药理学；心肌肥厚细胞；RhoA；ERK |
| Title： | Interaction between RhoA/ROCK pathway and ERK pathway in cultured hypertrophy cardiomyocyts |
| Author： | LI Le, ZHANG Ke, TAO Houquan, SHANG Hao, ZHANG Cailing |
| Organization： | College of Pharmaceutical Science, Zhejiang University of Technology; Central Lab, Zhejiang Province People’s Hospital; Rencai College of Zhejiang |
| Abstract： | Objective: To investigate whether the interaction between RhoA (ras homologue A)/ROCK pathway and extra cellular signal regulated protein kinase (ERK) pathway in the cultured hypertrophy cardiomyocyts. Methods: The hearts of neonatal rats were isolated and cultured in vitro，then the cardiomyocyts were divided into four groups which stimulated by same dosage isoprenaline respectively: control group, RhoA group, ERK blocking drug (PD98059) group, RhoA+ERK blocking drug (PD98059) group. The expression of ROCK, ERK1/2 proteins, the content of phosphorylated ERK1/2 (p-ERK1/2) and ROCK proteins (p-ROCK) were detected by Western blotting，and the expression of ROCK mRNA in the cultured hypertrophy cardiomyocyts was detected by reverse transcription PCR (RT-PCR). Results: Compared with the control group, the contents of p-ROCK and p-ERK1/2 proteins in RhoA group were increased (P<0.05), while those in ERK blocking drug group were increased (P<0.05). There was no difference between control group and RhoA+ERK blocking drug group. Compared with RhoA group, the contents of p-ROCK and p-ERK1/2 proteins in ERK blocking agent group and RhoA+ERK blocking drug group were decreased (P<0.05). There was no difference in the expression of ROCK and ERK1/2 proteins among different groups. There were obvious differences in expression of ROCK mRNA among different groups. Conclusion: RhoA may directly activate ROCK proteins and ERK1/2 pathway, and the ERK1/2 pathway may be located in the downstream of the RhoA/ROCK pathway. The activation of ROCK proteins may partially depend on ERK1/2 pathway stimulation. |
| Key words： | pharmacology; cardiac hypertrophy cell; RhoA; ERK |
| 发表期数： | 2016年3月第5期 |
| 引用格式： | 李乐，张可，陶厚权，等. 培养的肥厚心肌细胞中RhoA/ROCK通路和ERK通路的相互对话[J]. 中国科技论文在线精品论文，2016，9（5）：470-475. |
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