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长白红景天内生真菌抗氧化活性评价
发表时间:2016-03-15 浏览量:1721 下载量:699
全部作者: | 崔晋龙,郭婷婷,王梦亮,王俊宏,任振兴 |
作者单位: | 山西大学应用化学研究所;山西大学生物技术研究所 |
摘 要: | 通过发酵培养真菌,对发酵液的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、 "OH"自由基、O2" "自由基、亚硝酸盐清除能力以及Fe2+螯合能力进行系统测定,综合评价长白红景天内生真菌的抗氧化能力。结果发现,对DPPH自由基、 "OH"自由基、O2" "自由基、亚硝酸盐清除率以及Fe2+螯合率大于50%的内生真菌菌株分别占供试菌株的84%、74%、58%、4%和30%. 菌株Ra-R-32抗氧化活性较高,在5种评价中各清除率均大于50%. 实验表明,长白红景天内生真菌具有良好的抗氧化活性,是珍贵的化合物筛选资源库。 |
关 键 词: | 药用植物学;长白红景天;内生真菌;抗氧化;清除率 |
Title: | Screening of antioxidant activity of endophytic fungi from Rhodiola angusta |
Author: | CUI Jinlong, GUO Tingting, WANG Mengliang, WANG Junhong, REN Zhenxing |
Organization: | Institute of Applied Chemistry, Shanxi University; Institute of Biotechnology, Shanxi University |
Abstract: | To evaluate the antioxidant capacities of endophytic fungi from Rhodiola angusta systematically, scavenging activities to1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl free radical, ferrous ions, superoxide anion free radical and nitrite free radical were determined to fungal fermentation. The results showed that 84%, 74%, 58%, 4% and 30% of isolates that had the scavenging rate more than 50%. Ra-R-32 was a promising isolate with scavenging rate more than 50% in each evaluation method. The represent research indicated that endophytic fungi from R. angusta had remarkable antioxidant capacities, which could be potential resource of exploring novel chemicals. |
Key words: | medicinal botany; Rhodiola angusta; endophytic fungi; antioxidant; scavenging rate |
发表期数: | 2016年3月第5期 |
引用格式: | 崔晋龙,郭婷婷,王梦亮,等. 长白红景天内生真菌抗氧化活性评价[J]. 中国科技论文在线精品论文,2016,9(5):491-497. |
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