您的位置：首页 > 论文页面
钩端螺旋体对数生长期及毒力测定
发表时间：2016-05-15 浏览量：1942 下载量：695
| 全部作者： | 谢旭峰，张文龙，曹永国 |
| 作者单位： | 吉林大学动物医学学院 |
| 摘 要： | 目的：了解钩端螺旋体的生长规律和毒力，为下一步研究做准备。方法：使用EMJH液体培养基，在28～30℃条件下振荡培养，每隔6 h用细菌计数板检测钩体的浓度；取4～6周龄的雌性仓鼠，每组4只，腹腔注射不同浓度对数生长期的钩体，记录仓鼠的半数死亡天数；另取3组仓鼠，腹腔注射106对数生长期的钩体，检测钩体临4菌株的全致死量，并取感染仓鼠的肾脏、肝脏和肺脏，进行组织病理学观察。结果：1）钩体在0～150 h内呈S型增长，其中0～60 h内生长缓慢，80～105 h内生长迅速，130～150 h则进入稳定期。2）腹腔注射102～105钩体的仓鼠都有半数死亡，但都没有出现全致死，而腹腔注射106钩体的3组仓鼠，都在5～7 d出现了稳定的100%死亡率。3）组织病理学观察感染后的仓鼠肾小管和肺出血明显，肝细胞紧密连接增宽。结论：钩体临4菌株在EMJH培养基，28～30℃条件下培养，对数生长期为80～105 h；该菌株在4～6周龄仓鼠上的半数致死量为<102，全致死量为106，感染仓鼠的肾脏、肝脏和肺脏都出现明显的病理学损伤。 |
| 关 键 词： | 家畜传染学；钩端螺旋体；对数生长期；半数致死量；病理损伤 |
| Title： | Measurement of logarithmic phase and toxicity in Leptospira |
| Author： | XIE Xufeng, ZHANG Wenlong, CAO Yongguo |
| Organization： | College of Veterinary Medicine, Jilin University |
| Abstract： | Objective: To explore the growth characteristics and toxicity of leptospira for the next research. Methods: Leptospira was incubated in EMJH medium using a shaker at 28-30℃ and used bacteria count plate and dark-field microscope to measure the viable count every 6 h. Being chosed 4 groups of 4 to 6-week-old female golden hamsters, each group having four hamsters. Intraperitoneal challenge of hamster with different concentrations’ leptospiroses, keeping a record of the days these hamsters died in half. Besides, being chosed 3 groups of hamsters, intraperitoneally challenged 106 leptospiroses living in logarithmic phase, measuring the lethal dose and geted the infected hamsters’ kidneys, livers and lungs to do histopathological examination. Results: 1) During 0-150 h, Leptospira growth showed S curve. During 0 to 60 h, it growed slowly, developed rapidly in 80-105 h but in 130-150 h came into stable phase. 2) Intraperitoneally challenging hamsters of 102-105 Leptospira all died an half but 3 groups of hamsters intraperitoneally challenged 106 all died in 5-7 d. 3) Histopathological examination showed that infected hamsters’ lungs bled obviously, livers’ cells tight coupling broadened, kidney tubules also bled. Conclusion: The logarithmic phase of Leptospira was 80-105 h in EMJH medium at 28-30℃. The medium lethal dose of Leptospira in 4 to 6-week-old female golden hamster was 102, lethal dose was 106. Infected hamsters showed obviously pathological damage. |
| Key words： | animal molysmology; Leptospira; logarithmic phase; LD50; pathology |
| 发表期数： | 2016年5月第9期 |
| 引用格式： | 谢旭峰，张文龙，曹永国. 钩端螺旋体对数生长期及毒力测定[J]. 中国科技论文在线精品论文，2016，9（9）：968-973. |
2
评论数 0请您登录
暂无评论
下载全文
多维论文