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龙牙楤木皂苷IV对心肌细胞缺氧/复氧损伤的保护作用
发表时间：2016-06-15 浏览量：2007 下载量：842
| 全部作者： | 杨明华，刘影，王敏，孙桂波 |
| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究中心；中国医学科学院北京协和医学院药用植物研究所 |
| 摘 要： | 目的：观察龙牙楤木皂苷IV对缺氧/复氧（hypoxia/reoxygenation，H/R）诱导H9c2心肌细胞损伤的保护作用。方法：实验分为5组，分别为空白对照组（C组），缺氧/复氧模型组（H/R组），缺氧/复氧模型+龙牙楤木皂苷IV低（12.5 mg/L）、中（25.0 mg/L）、高（50.0 mg/L）剂量组。利用缺氧/复氧的方法诱导体外培养的H9c2心肌细胞损伤来模拟临床上心肌缺血再灌注损伤（myocardial ischemia/reperfusion injury，MIRI），观察龙牙楤木皂苷IV对缺氧/复氧所致H9c2心肌细胞损伤的保护作用。MTT法检测细胞的存活率；Hoechst 33342/PI荧光染色法观察龙牙楤木皂苷IV对缺氧/复氧诱导心肌细胞凋亡的影响以及JC-1荧光探针法检测H9c2心肌细胞线粒体膜电位的变化。结果：与空白对照组相比，H/R组细胞存活率显著降低（P<0.01）；与H/R组相比，龙牙楤木皂苷IV各剂量组细胞存活率和线粒体膜电位明显升高（P<0.05），并且心肌细胞凋亡率显著降低（P<0.05）。结论：龙牙楤木皂苷IV对心肌细胞缺氧/复氧损伤有明显的保护作用，呈剂量依赖性，其作用机制可能与降低心肌细胞凋亡、升高线粒体膜电位有关。 |
| 关 键 词： | 药效学；龙牙楤木皂苷IV；缺氧/复氧；H9c2心肌细胞 |
| Title： | Protective effects of Tarasaponin IV on hypoxia/reoxygenation injury in H9c2 cardiocytes |
| Author： | YANG Minghua, LIU Ying, WANG Min, SUN Guibo |
| Organization： | Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College |
| Abstract： | Objective: To investigate protective effects of Tarasaponin IV on H9c2 cardiac myocytes exposed to simulated hypoxia/reoxygenation (H/R). Methods: This experiment were divided into five groups: normal control group (group C); hypoxia/reoxygenation (H/R group) model group; hypoxia/reoxygenation model + Tarasaponin IV low (12.5 mg/L), medium (25.0 mg/L), high (50.0 mg/L) dose group. We use of cardiac H9c2 cells cultured in vitro. Using hypoxia/reoxygenation method of myocardial cell injury induced H9c2 to simulate the clinical myocardial ischemia/reperfusion injury (MIRI), we observe Tarasaponin IV of the hypoxia protective effects of H9c2 myocardial cell injury. Tarasaponin IV intervention by MTT assay cell viability; Hoechst 33342/PI fluorescence staining Tarasaponin IV on hypoxia/reoxygenation-induced apoptosis of myocardial cells and JC-1 fluorescent probe assay H9c2 mitochondrial membrane potential. Results: Compared with the control group, H/R group cell viability was significantly decreased (P<0.01). Compared with the H/R group, cell viability and mitochondrial membrane potential was significantly increased (P<0.05) in Tarasaponin IV group, and myocardial cell apoptosis was significantly reduced (P<0.05). Conclusion: Tarasaponin IV on myocardial hypoxia/reoxygenation injury has a significant protective effect in a dose-dependent manner. The mechanism may be associated with decreased myocardial apoptosis, increased mitochondrial membrane potential. |
| Key words： | pharmacodynamics; Tarasaponin IV; hypoxia/reoxygenation; H9c2 cardiocytes |
| 发表期数： | 2016年6月第11期 |
| 引用格式： | 杨明华，刘影，王敏，等. 龙牙楤木皂苷IV对心肌细胞缺氧/复氧损伤的保护作用[J]. 中国科技论文在线精品论文，2016，9（11）：1115-1120. |
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