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中空纤维/分子印迹复合电极的制备及其在痕量多巴胺直接分析中的应用研究

发表时间:2016-06-15  浏览量:2116  下载量:900
全部作者: 李兵兵,周峰,黄凯,荆涛
作者单位: 华中科技大学同济医学院公共卫生学院,教育部、环保部环境与健康重点实验室,省部共建环境卫生学国家重点实验室培育基地
摘 要: 目的:构建中空纤维/分子印迹复合电极,并将其应用于细胞培养基中痕量多巴胺的快速、直接分析,为深入研究多巴胺的作用机理提供必要的技术支持。方法:以绿色、环保的尼克酰胺为功能单体,在铂电极表面修饰出分子印迹膜,实现对多巴胺的高选择性识别,并将电极插入到一端热封的中空纤维管中,制备出集萃取/富集/检测一体化复合电极,同步实现高效萃取、选择性富集乃至快速检测。结果:复合电极对多巴胺表现出高选择性识别能力,不会受到共存干扰物和样本基体的干扰,方法的检测限为8 nmol/L,回收率在95.1%~109.2%之间,相对标准偏差(relative standard deviation,RSD)不高于9.6%. 结论:中空纤维/分子印迹复合电极能够实现对多巴胺的快速、直接、无损分析,在疾病诊断、药物治疗及基础研究等方面具有广阔的实际应用前景。
关 键 词: 卫生检验学;中空纤维;分子印迹膜;电化学传感器;多巴胺
Title: Preparation of hollow fiber-supported molecularly imprinted sensors and its application in the direct determination of dopamine in serum samples
Author: LI Bingbing, ZHOU Feng, HUANG Kai, JING Tao
Organization: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology
Abstract: Objective: Hollow fiber-supported molecularly imprinted sensors were developed for the direct determination of dopamine in cell culture medium, which provided an effective technology to study the mechanism of dopamine. Methods: Molecularly imprinted membrance on the surface of electrode was firstly prepared by using nicotinamide as an environment-friendly monomer to selectively recognize the template molecules. Then, this electrode was attached inside the pores of hollow fibers to prepare an integrated electrode for extraction, concentration and then determinationof dopamine in cell culture medium, simultaneously. Results: Hollow fiber-supported molecularly imprinted composites showed highly selective recognition for dopamine without the interference of ascorbic acid and uric acid coexisting biological fluids. The linear range was changed from 20 nmol/L to 15 μmol/L and the detection limit was 8 nmol/L. The spiked recoveries were changed from 95.1% to 109.2% and the relative standard deviation (RSD) was not higher than 9.6%. Conlusion: Hollow fiber-supported molecularly imprinted sensors can be used for selective, sensitive and direct determination of dopamine in complex samples, which exhibited significant promise in disease diagnosis, medication and fundamental research.
Key words: sanitary inspection; hollow fiber; molecularly imprinted membrance; electrochemical sensor; dopamine
发表期数: 2016年6月第11期
引用格式: 李兵兵,周峰,黄凯,等. 中空纤维/分子印迹复合电极的制备及其在痕量多巴胺直接分析中的应用研究[J]. 中国科技论文在线精品论文,2016,9(11):1148-1154.
 
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