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吸入不同时间异氟烷对大鼠发育期海马组织β-catenin表达的影响
发表时间：2016-09-15 浏览量：1656 下载量：385
| 全部作者： | 孙丽宁，孙越，齐军，王雪芹，李克忠 |
| 作者单位： | 山东大学第二医院麻醉科；烟台毓璜顶医院麻醉科；临沂市人民医院麻醉科 |
| 摘 要： | 目的：观察吸入不同时间异氟醚对发育期大鼠海马组织β-catenin蛋白表达的影响。方法：出生后7 d雄性SD大鼠32只，随机分为4组：吸入空气的对照组（A组），吸入2%异氟醚2 h组（B组），吸入2%异氟醚4 h组（C组），以及吸入2%异氟醚6 h组（D组），每组幼鼠处理中均无缺氧及CO2蓄积发生。实验结束6 h后，将上述各组幼鼠随机分为RT-PCR实验组和Western blotting实验组，分别取海马组织检测β-catenin蛋白表达。结果：发育期SD大鼠海马组织β-catenin mRNA的表达与对照组相比，B组和D组有统计学差异（P |
| 关 键 词： | 麻醉学；吸入麻醉；异氟醚；海马组织；β-catenin |
| Title： | Effects of different time course of isoflurane on β-catenin expression in the hippocampus of developing rats |
| Author： | SUN Lining, SUN Yue, QI Jun, WANG Xueqin, LI Kezhong |
| Organization： | Department of Anethesiology, The Second Hospital of Shandong University; Department of Anethesiology, Yantai Yuhuangding Hospital; Department of Anethesiology, Linyi People’s Hospital |
| Abstract： | Objective: To compare effects of different time course of isoflurane on β-catenin expression in the hippocampus of developing rats. Methods: Thirty-two 7-day-old SD male rats were randomly divided into four groups: control group inhalation of O2 instead of isoflurane (A), inhalation of 2% isoflurane 2 h group (B) , inhalation of 2% isoflurane 4 h group (C) and inhalation of 2% isoflurane 6 h group (D). Rats in every group were no hypoxia and CO2 accumulation occurred in the process. 6 h after treatment rats were killed and randomly divided into RT-PCR group and Western blotting group. Their hippocampuses were removed immediately to detect the expression of β-catenin protein. Results: In the group of PCR, compared with control group, the level of β-catenin was increased markedly (P |
| Key words： | anesthesiology; inhalation anesthesia; isoflurane; hippocampus; β-catenin |
| 发表期数： | 2016年9月第17期 |
| 引用格式： | 孙丽宁，孙越，齐军，等. 吸入不同时间异氟烷对大鼠发育期海马组织β-catenin表达的影响[J]. 中国科技论文在线精品论文，2016，9（17）：1785-1788. |
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