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HPLC-ELSD法测定墨旱莲中旱莲苷A与旱莲苷Ⅰ的含量
发表时间：2016-09-15 浏览量：1696 下载量：393
| 全部作者： | 潘 晴，马鲁豫，田景奎 |
| 作者单位： | 山东省皮肤病医院；山东省医学科学院科研处；浙江大学生物医学工程与仪器科学学院 |
| 摘 要： | 目的：研究墨旱莲皂苷的质量标准。方法：对5个不同产地的墨旱莲药材进行性状鉴别，测定其水分和浸出物的含量；应用高效液相色谱（high performance liquid chromatography，HPLC）-蒸发光散射检测器（evaporative light-scattering detector，ELSD），建立墨旱莲皂苷含量的测定方法，按照20倍量70%乙醇，回流提取5次，合并提取液，浓缩，测定含量。结果：水分含量符合药典规定；70%乙醇浸出物含量定为不低于20.0%；墨旱莲总皂苷以旱莲苷A与旱莲苷Ι含量之和计，不得低于0.45%. 结论：本研究对墨旱莲皂苷的质量标准做了探讨，为墨旱莲皂苷的进一步开发奠定基础。 |
| 关 键 词： | 中药化学；墨旱莲皂苷；质量标准；水分；浸出物 |
| Title： | Quality standard determination of ecliptasaponin A and ecliptasaonin Ι from Eclipta using HPLC-ELSD |
| Author： | PAN Qing, MA Luyu, TIAN Jingkui |
| Organization： | Shandong Demartological Hospital; Scientific Research Office, Shandong Academy of Medical Science; College of Biomedical Engineering & Instrument Science, Zhejiang University |
| Abstract： | Objective: To research the quality standards for saponin from the Eclipta. Methods: we identified the characteristics of the Fructus ligustri lucidi from 5 different producing areas, and determined their contents of the moisture and the extraction. We also use the high performance liquid chromatography (HPLC)-evaporative light-scattering detector (ELSD) to establish the method of determining the saponin from Eclipta. We applied the twenty times of 70% ethanol to extract total saponin from the 5 different producing areas’ Eclipta 5 times. Then we merged, enriched the extract and determined the total saponin content. Results: The content of moisture is conformed to the Pharmacopoeia of the People’s Republic of China. The content of the 70% ethanol extraction couldn’t be less than 20%. When the content of the saponin from Eclipta is in the equivalent of ecliptasaponin A and ecliptasaonin Ι, the result couldn’t be less than 0.45%. Conclusion: This study replenished the quality standard of the contents of saponin from Eclipta, which laid a foundation for its further development. |
| Key words： | chemistry of Chinese medicine; saponin from Eclipta; quality standard; moisture; extraction |
| 发表期数： | 2016年9月第17期 |
| 引用格式： | 潘 晴，马鲁豫，田景奎. HPLC-ELSD法测定墨旱莲中旱莲苷A与旱莲苷Ⅰ的含量[J]. 中国科技论文在线精品论文，2016，9（17）：1810-1814. |
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