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GABA通过抑制内质网未折叠蛋白反应对抗酒精的肝毒性作用
发表时间：2016-12-15 浏览量：2019 下载量：379
| 全部作者： | 刘艳丽，罗丹，李韬，王双连 |
| 作者单位： | 山东省肿瘤医院基础研究中心；山东大学医学院 |
| 摘 要： | 研究采用噻唑蓝（MTT）实验和Western blotting等方法，探讨了伽马氨基丁酸（gamma-aminobutyric acid，GABA）是否通过内质网（endoplasmic reticulum，ER）未折叠蛋白反应（unfolded protein response，UPR）发挥对抗酒精对肝细胞的增殖抑制作用。结果发现，酒精对体外培养的BRL肝细胞的增殖抑制作用可被GABA逆转，且酒精诱导的UPR标志物GRP78和CHOP表达上调被GABA抑制。以上结果说明GABA抑制酒精的肝毒性作用与GABA抑制酒精诱导的UPR有关。 |
| 关 键 词： | 人体生理学；肝脏生理；酒精性肝病；伽马氨基丁酸；未折叠蛋白反应；增殖 |
| Title： | Protectiverole of GABA against ethanol hepatotoxicity involves inhibition of endoplasmic reticulum unfolded protein response |
| Author： | LIU Yanli, LUO Dan, LI Tao, WANG Shuanglian |
| Organization： | Basic Research Center, Shandong Tumor Hospital; School of Medicine, Shandong University |
| Abstract： | Using MTT and Western blotting analysis, whether unfolded protein response (UPR) of endoplasmic reticulum (ER) involves in the cytoprotective effect of gamma-aminobutyric acid (GABA) against ethanol-induced hepatotoxicity was investigated. The results showed that ethanol-induced decrease of cell viability was inhibited by GABA treatmentin cultured BRL hepatocytes. GABA inhibited ethanol-induced up-regulated expression of GRP78 and CHOP, which are UPR markers. These results suggest that inhibition of UPR is involved in the protective role of GABA against ethanol hepatotoxicity. |
| Key words： | human physiology; liver physiology; alcoholic liver disease; gamma-aminobutyric acid; unfolded protein response; proliferation |
| 发表期数： | 2016年12月第23期 |
| 引用格式： | 刘艳丽，罗丹，李韬，等. GABA通过抑制内质网未折叠蛋白反应对抗酒精的肝毒性作用[J]. 中国科技论文在线精品论文，2016，9（23）：2445-2449. |
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