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混合菌群DDMY1对活性黑5的脱色降解研究

发表时间:2016-12-31  浏览量:1632  下载量:655
全部作者: 谢学辉
作者单位: 东华大学环境科学与工程学院
摘 要: 利用梯度浓度压力驯化法,从运行良好的印染废水水解酸化反应器中筛选出对染料活性黑5具有良好脱色性能的混合菌群DDMY1,利用该菌群在兼氧条件下对活性黑5进行脱色研究。研究表明,混合菌群DDMY1对活性黑5的脱色性能显著,400 mg/L活性黑5染料经过24 h培养,脱色率能达到97.4%. 运用紫外—可见全波长扫描分光光度法检测不同时间、不同状态下脱色液吸光值情况。运用气相色谱—质谱联用(gas chromatography-mass spectrometer,GC-MS)和傅里叶转变红外光谱(Fourier transform infrared spectroscopy,FTIR)等检测方法分析生物脱色过程中的产物情况,检测到含有芳环或酰胺结构的物质可能是活性黑5的生物降解产物,从染料结构变化的角度揭示活性黑5生物脱色降解过程。
关 键 词: 环境生物学;混合菌群;染料脱色;染料降解;活性黑5
Title: Decolorization and degradation of reactive black 5 by mixed flora DDMY1
Author: XIE Xuehui
Organization: College of Environmental Science & Engineering, Donghua University
Abstract: In this paper, by using the concentration gradient pressure screening method, a mixed bacterial flora DDMY1, which has good decolorization performance on reactive black 5, was selected from the well-runnig hydrolytic acidification reactor for printing and dyeing wastewater treatment. The bacterial flora was used to decolor reactive black 5 under the facultative-aerobic condition. Results showed that the mixed flora DDMY1 performed significantly in decoloring of reactive black 5, with 400 mg/L reactive black 5 for 24 h cultivation, the decolorization rate could reach up to 97.4%. Using ultraviolet-visible spectrophotometry method scanned the decoloring liquid at different time and different states. Using gas chromatography-mass spectrometer (GC-MS) and the Fourier transform infrared spectroscopy (FTIR) methods analyzed the degradation products in the process of decolorization. It was found that the substance containing the aromatic ring or the amide structure may be the biodegradation products of reactive black 5. The results in this study were expected to reveal the mechanism of the decolorization phenomenon from the aspect of the dye’s structure changing.
Key words: environmental biology; mixed flora; dye decolorization; dye degradation; reactive black 5
发表期数: 2016年12月第24期
引用格式: 谢学辉. 混合菌群DDMY1对活性黑5的脱色降解研究[J]. 中国科技论文在线精品论文,2016,9(24):2564-2571.
 
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