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检测布氏杆菌抗体直接阻断ELISA方法的建立
发表时间：2017-02-16 浏览量：2107 下载量：513
| 全部作者： | 吴雯，朱桥平，王超凡，宰栩生，邵红霞，叶建强，钱琨，秦爱建 |
| 作者单位： | 扬州大学兽医学院，省部共建禽病预防医学教育部重点实验室；扬州大学，江苏省动物重要疫病与人兽共患病防控协同创新中心；扬州大学兽医学院，江苏省人兽共患病学重点实验室 |
| 摘 要： | 利用研制的抗布氏杆菌特异性单克隆抗体5C10建立了检测血清中布氏杆菌抗体的直接阻断酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）方法，并对该方法反应条件进行优化，组建试剂盒。用该试剂盒与进口ELISA试剂盒对已知背景的75份牛血清和25份羊血清样品进行检测比较。结果显示，本研究建立的检测布氏杆菌病血清抗体直接阻断ELISA方法的特异性和敏感性好，且结果稳定，与进口试剂盒检测结果符合率达到100%. 该试剂盒将在我国布氏杆菌病的净化与根除工作中具有重要应用价值。 |
| 关 键 词： | 兽医学；布氏杆菌病；单克隆抗体；阻断ELISA |
| Title： | Development of directly blocking enzyme-linked immunosorbent assay for antibodies against brucellosis |
| Author： | WU Wen1, ZHU Qiaoping1, WANG Chaofan1, ZAI Xusheng2, 3, SHAO Hongxia1, 2, 3, YE Jianqiang1, 2, 3, |
| Organization： | Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University; Jiangsu Key Laboratory of Zoonosis, College of Veterinary Medicine, Yangzhou University |
| Abstract： | A directly blocking enzyme-linked immunosorbent assay (ELISA) to detect the antibodies against brucellosis was developed with monoclonal antibody (mAb) 5C10 to Brucella abortus. Main factors related to the effect of blocking ELISA reaction were optimized and a kit was made. 75 Bovine sera samples and 25 sheep sera samples were detected by both our kit and commercial ELISA kit. Results showed that the detection coincidence rate was 100%. Directly blocking ELISA to test sera against brucellosis is very sensitive, specific and stable. It will be very important and useful in the eradication of brucellosis in China. |
| Key words： | veterinary science; brucellosis; monoclonal antibody; blocking ELISA |
| 发表期数： | 2017年2月第3期 |
| 引用格式： | 吴雯，朱桥平，王超凡，等. 检测布氏杆菌抗体直接阻断ELISA方法的建立[J]. 中国科技论文在线精品论文，2017，10（3）：332-340. |
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