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HGF通过激活ERK信号通路减弱TGF-β1的促纤维化作用
发表时间：2017-06-15 浏览量：1876 下载量：400
| 全部作者： | 韩沛林，崔清波，范美丽，陆文君，李洲，李昭铸 |
| 作者单位： | 哈尔滨医科大学第二临床医学院小儿外科 |
| 摘 要： | 目的：探究肝细胞生长因子（hepatocyte growth factor，HGF）降低转化生长因子-β1（transforming growth factor-β1，TGF-β1）诱导纤维化的作用和可能机制。方法：使用不同浓度的HGF对体外培养的大鼠成纤维细胞进行预处理，随后进行TGF-β1的纤维化诱导，检测诱导后肌腱成纤维细胞的纤维连接蛋白（fibronectin）及α-平滑肌肌动蛋白（alpha smooth muscle actin，α-SMA）的表达及细胞外调节蛋白激酶（extracelluar regulated protein kinase，ERK）磷酸化情况。随后阻断ERK信号通路，再次观察fibronectin及α-SMA的表达情况。结果：HGF可以减弱TGF-β1诱导纤维化的作用。当阻断ERK信号通路时，这种中和作用明显减弱。结论：本实验证实HGF可通过ERK信号通路减弱TGF-β1对肌腱成纤维细胞的纤维化作用。 |
| 关 键 词： | 外科学；肝细胞生长因子；转化生长因子-β1；纤维化 |
| Title： | HGF attenuating the fibrosis of TGF-β1 by activating ERK signaling pathway |
| Author： | HAN Peilin, CUI Qingbo, FAN Meili, LU Wenjun, LI Zhou, LI Zhaozhu |
| Organization： | Pediatric Department, The Second Affiliated Hospital of Harbin Medical University |
| Abstract： | Objective: We explore the role of hepatocyte growth factor (HGF) in reducing transforming growth factor-β1 (TGF-β1)-induced fibrosis and the possible mechanism. Methods: The fibroblasts cultured in vitro were pretreated with different concentrations of HGF. The fibrosis induction of TGF-β1 was performed and the expression of fibronectin and α-smooth muscle actin (α-SMA) in the induced tendon fibroblasts and the phosphorylation of extracelluar regulated protein kinase (ERK) were detected. Followed by blocking the ERK signaling pathway, then we observed fibronectin and α-SMA expression. Results: HGF can attenuate the effect of TGF-β1 on fibrosis, and this neutralization is significantly attenuated when the ERK signaling pathway is blocked. Conclusion: This study confirms that HGF can reduce the fibrosis of TGF-β1 on tendon fibroblasts by ERK signaling pathway. |
| Key words： | surgery; hepatocyte growth factor; transforming growth factor-β1; fibrosis |
| 发表期数： | 2017年6月第11期 |
| 引用格式： | 韩沛林，崔清波，范美丽，等. HGF通过激活ERK信号通路减弱TGF-β1的促纤维化作用[J]. 中国科技论文在线精品论文，2017，10（11）：1196-1202. |
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