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日本血吸虫可溶性成虫抗原及可溶性虫卵抗原对肝星状细胞（LX2）影响的比较研究
发表时间：2017-06-15 浏览量：2132 下载量：427
| 全部作者： | 王立富，余子龙，解辉，吴忠道，孙希 |
| 作者单位： | 中山大学中山医学院寄生虫学教研室，热带病防治研究教育部重点实验室 |
| 摘 要： | 目的：通过用可溶性成虫抗原（soluble worm antigen，SWA）和可溶性虫卵抗原（soluble egg antigen，SEA）刺激肝星状细胞（LX2），分析比较SWA、SEA对人LX2细胞的影响，从而探究日本血吸虫感染所致肝纤维化的发病机理。方法：利用SWA、SWA+转化生长因子-β（transforming growth factor-β，TGF-β）、SEA、SEA+TGF-β、TGF-β分别刺激LX2细胞，实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）检测与LX2细胞活化相关的重要基因α-SMA、Col1a1、CTGF、PPARγ、MMP9、MMP2、CCL2的表达情况。结果：SWA刺激LX2细胞后，α-SMA、Col1a1、CTGF升高，与TGF-β具有协同作用；而SEA刺激LX2细胞后，α-SMA、Col1a1、CTGF下降，MMP9、CCL2升高，并且抑制TGF-β对LX2细胞的作用。结论：SWA具有促进肝纤维化、基质重建的作用；而SEA具有抑制肝纤维化、基质重建和调节炎症介质的作用。本研究为阐明日本血吸虫感染所致肝纤维化的发生发展机制提供了理论基础。 |
| 关 键 词： | 医学寄生虫学；日本血吸虫；可溶性成虫抗原（SWA）；可溶性虫卵抗原（SEA）；LX2细胞 |
| Title： | Effects comparison between Schistosoma japonicum soluble worm antigen and soluble egg antigen on hepatic stellate cells (LX2) |
| Author： | WANG Lifu, YU Zilong, XIE Hui, WU Zhongdao, SUN Xi |
| Organization： | Key Laboratory of Tropical Diseases Control, Ministry of Education, Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University |
| Abstract： | Objective: By stimulating LX2 cells with soluble worm antigen (SWA) and soluble egg antigen (SEA) and comparing the effects of SWA and SEA on LX2 cells, we investigated the mechanism of hepatic fibrosis when infected by Schistosoma japonicum. Methods: After treating LX2 cells with SWA, SWA+transforming growth factor-β (TGF-β), SEA, SEA+TGF-β, TGF-β, the LX2 cells activation related genes (α-SMA, Col1a1, CTGF, PPARγ, MMP9, MMP2, CCL2) were investigated by quantitative real-time PCR (qRT-PCR). Results: SWA treatment on LX2 cells results in the increases in the expression of α-SMA, Col1a1, CTGF and has synergistic effect with TGF-β, while treating with SEA results in the decreases in the expression of α-SMA, Col1a1, CTGF and increases in MMP9, CCL2. SEA can inhibit the effect of TGF-β on LX2 cells. Conclusion: SWA has the effects of promoting hepatic fibrosis and matrix remodelling, while SEA can inhibite hepatic fibrosis, matrix remodelling and regulate inflammatory mediator. These results provide theoretical basis on illustrating the development of hepatic fibrosis induced by S. japonicum infection. |
| Key words： | medical parasitology; Schistosoma japonicum; SWA; SEA; LX2 cells |
| 发表期数： | 2017年6月第11期 |
| 引用格式： | 王立富，余子龙，解辉，等. 日本血吸虫可溶性成虫抗原及可溶性虫卵抗原对肝星状细胞（LX2）影响的比较研究[J]. 中国科技论文在线精品论文，2017，10（11）：1255-1260. |
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