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胜红蓟黄脉病病原的分子鉴定
发表时间：2017-11-15 浏览量：2022 下载量：278
| 全部作者： | 陈家盛，胡汝检，朱重庆，林文武，林文忠，林丹，张洁 |
| 作者单位： | 福建农林大学植物保护学院，植物病毒研究所，福建省植物病毒学重点实验室 |
| 摘 要： | 对福建农林大学校内表现黄花叶、明脉等疑似感染双生病毒症状的胜红蓟进行病原鉴定。利用双生病毒特异性简并引物PA/PB扩增得到500 bp的DNA-A部分片段，再根据已获得的片段设计特异性引物将其全长剩余部分约2.3 kb扩增出来，序列拼接得到全长2 734 nts. 序列分析发现，该病毒与台湾一种胜红蓟黄脉病毒的同源性高达99.5%. 同时，利用引物β01/β02扩增出DNA-β卫星的全长（1 346 nts）。序列分析发现，此卫星分子与一种胜红蓟黄脉病毒伴随的卫星分子的同源性最高，达 98.1%. 因此，判断此种病毒为胜红蓟黄脉病毒的一种分离物。 |
| 关 键 词： | 植物病毒学；双生病毒；胜红蓟；DNA-A；DNA-β |
| Title： | Molecular identification of the pathogen inducing Ageratum yellow vein disease |
| Author： | CHEN Jiasheng, HU Rujian, ZHU Chongqing, LIN Wenwu, LIN Wenzhong, LIN Dan, ZHANG Jie |
| Organization： | Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University |
| Abstract： | In this study, Ageratum samples showing obvious yellow mosaic leaves and vein clearing geminiviral-like symptoms were selected in campus of Fujian Agriculture and Forestry University. By using the degenerate primers PA/PB for geminivirus, a partial DNA-A sequence with 500 bp was amplified. Then, using designed specific primers, the left sequence (around 2.3 kb) of the full-length was obtained. Sequence splicing showed the full length of 2 734 nts. Sequence analysis showed that this virus had a high sequence identity of 99.5% with Ageratum yellow vein virus isolated from Taiwan. Meanwhile, using primers β01/β02 for the betasatellite, the full-length of DNA-β was amplified, which was 1 346 nts. Sequence analysis showed that this betasatellite had a high sequence identity of 98.1% with an Ageratum yellow vein virus betasatellite. Therefore, the virus identified in this study was considered as an isolate of Ageratum yellow vein virus. |
| Key words： | plant virology; geminivirus; Ageratum conyzoides; DNA-A; DNA-β |
| 发表期数： | 2017年11月第21期 |
| 引用格式： | 陈家盛，胡汝检，朱重庆，等. 胜红蓟黄脉病病原的分子鉴定[J]. 中国科技论文在线精品论文，2017，10（21）：2398-2403. |
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