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纤维母细胞高表达VEGF-C在头颈部肿瘤侵袭转移中的作用
发表时间：2017-12-15 浏览量：2555 下载量：535
| 全部作者： | 洪筠，曾柏瑞，陈昕，严旭彤，刘海雯 |
| 作者单位： | 中山大学光华口腔医学院•附属口腔医院 |
| 摘 要： | 目的：研究纤维母细胞中血管内皮生长因子-C（vascular endothelial growth factor-C，VEGF-C）高表达与头颈部鳞癌肿瘤生长、转移的关系。方法：将带荧光素酶（Luciferase）基因的质粒转染到头颈部肿瘤细胞，带VEGF-C的质粒转染到成纤维3T3细胞，通过体内外实验，探索VEGF-C在头颈部鳞癌肿瘤生长与转移中发挥的作用及其可能机制。结果：Western blotting实验证明Luciferase基因已成功转染到头颈部肿瘤细胞中。酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）实验证明VEGF-C基因已成功转染到3T3细胞中。免疫荧光实验证明转染了VEGF-C的3T3细胞具备肿瘤相关性纤维母细胞（cancer-associated fibroblasts，CAFs）的某些特性。体内成瘤实验观察到两组大鼠体内肿瘤大小存在显著性差别。结论：VEGF-C通过作用于CAFs，在头颈部肿瘤的早期转移中起主导作用。 |
| 关 键 词： | 口腔医学；头颈部鳞状细胞癌；纤维母细胞；血管内皮生长因子-C |
| Title： | Role of high-expression VEGF-C of fibroblasts in head and neck squamous cell |
| Author： | HONG Yun, ZENG Bairui, CHEN Xin, YAN Xutong, LIU Haiwen |
| Organization： | Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University |
| Abstract： | Objective: To study the relationship between the high expression of vascular endothelial growth factor-C (VEGF-C) in fibroblasts and the growth and metastasis of head and neck squamous cell carcinoma. Methods: We transfected plasmid containing Luciferase gene into head and neck cancer cells, and transfected plasmid containing VEGF-C into 3T3 fibroblasts, through experiments in vivo and in vitro to explore the role of VEGF-C in the growth and metastasis of head and neck squamous cell carcinoma and its possible mechanism. Results: Western blotting experiments showed that Luciferase gene was successfully transfected into the head and neck cancer cells. Enzyme-linked immuno sorbent assay (ELISA) experiments showed that VEGF-C gene was successfully transfected into the 3T3 cells. The immunofluorescence tests showed that the 3T3 cells transfected with VEGF-C had some properties of cancer-associated fibroblasts (CAFs). There were significant differences in tumor size between the two groups of rats in vivo. Conclusion: VEGF-C plays a leading role in the early metastasis of head and neck tumors by acting on CAFs. |
| Key words： | stomatology; head and neck squamous cell carcinoma; fibroblast; vascular endothelial growth factor-C |
| 发表期数： | 2017年12月第23期 |
| 引用格式： | 洪筠，曾柏瑞，陈昕，等. 纤维母细胞高表达VEGF-C在头颈部肿瘤侵袭转移中的作用[J]. 中国科技论文在线精品论文，2017，10（23）：2600-2607. |
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