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新颖栽培丹参材料的rDNA内外转录间隔区（ITS和ETS）分子标记鉴定
发表时间：2023-09-27 浏览量：364 下载量：60
| 全部作者： | 尹艳艳，任瑞花，冯洁，李海宾，王锐钦，李关荣 |
| 作者单位： | 西南大学农学与生物科技学院；太原市第六十三中学 |
| 摘 要： | 本研究对前期发现的2个新颖多年生大紫花单株（V-HNYZ-bV-1和W-SXXA-bV-1）和1个二年生白花丹参（Salvia miltiorrhiza）栽培居群（W-SCHY-W-1）材料，进行了基于其rDNA内、外转录间隔区（ITS和ETS）的分子标记鉴定。结果表明：W-SXXA-bV-1和V-HNYZ-bV-1的ITS的GC含量均较高，W-SCHY-W-1的ITS和ETS的GC含量最低；新颖材料W-SXXA-bV-1和V-HNYZ-bV-1的ITS序列变异率较高，均高于W-SCHY-W-1，而对于ETS序列，W-SXXA-bV-1和V-HNYZ-bV-1接近（7.1%～6.5%），但低于W-SCHY-W-1（8.2%）；W-SXXA-bV-1与V-HNYZ-bV-1亲缘关系较近，W-SXXA-bV-1、V-HNYZ-bV-1和W-SCHY-W-1均与Q-DZH-V-4亲缘关系较远，V-HNYZ-V-1与Q-DZH-V-4亲缘关系很近。上述结果与栽培形态观察结果都高度一致表明：W-SXXA-V-1和V-HNYZ-V-1是康定或甘西鼠尾草，新颖单株材料W-SXXA-bV-1可能为W-SXXA-V-1的突变体，V-HNYZ-V-1和Q-DZH-V-4同为荔枝草，V-HNYZ-bV-1可能为混杂在V-HNYZ-V-1中的康定或西藏鼠尾草，与W-SXXA-bV-1非常相似。W-SCHY-W-1为粘毛鼠尾草。 |
| 关 键 词： | 分子生物学；栽培丹参；内转录间隔区（ITS）；外转录间隔区（ETS）；分子标记鉴定；遗传多样性 |
| Title： | Molecular marker identification of rDNA internal and external transcribed spacer (ITS and ETS) of novel cultivated Danshen materials |
| Author： | YIN Yanyan1, REN Ruihua, FENG Jie, LI Haibin, WANG Ruiqin, LI Guanrong |
| Organization： | College of Agronomy and Biotechnology, Southwest University; Taiyuan No. 63 Middle School |
| Abstract： | In this research, two novel perennial large purple-flowered monocots (V-HNYZ-bV-1 and W-SXXA-bV-1) and one biennial white-flowered Danshen (Salvia miltiorrhiza) in field cultivation (W-SCHY-W-1) were identified by molecular marker based on the internal and external transcribed spacers (ITS and ETS) of the rDNA. Results showed that the GC contents of ITS in W-SXXA-bV-1 and V-HNYZ-bV-1 were relatively high, while the GC contents of ITS and ETS in W-SCHY-W-1were the lowest. The ITS sequence variation rates of the novel materials W-SXXA-bV-1 and V-HNYZ-bV-1 was relatively high, and significantly higher than that of W-SCHY-W-1. The ETS sequence variation rates of W-SXXA-bV-1 and V-HNYZ-bV-1 were closer (7.1%-6.5%), but lower than that of the W-SCHY-W-1 (8.2%). The relationship between W-SXXA-bV-1 and V-HNYZ-bV-1 was closer, the relationship between W-SXXA-bV-1/V-HNYZ-bV-1/W-SCHY-W-1 and Q-DZH-V-4 was relative distant, and the relationship between V-HNYZ-V-1 and Q-DZH-V-4 was the closest. The results were highly consistent with field cultivational observation. It is suggested that W-SXXA-V-1 and V-HNYZ-V-1 are S. prattii or S. przewalskii. The novel single plant W-SXXA-bV-1 is likely a mutant of W-SXXA-V-1. Both V-HNYZ-V-1 and Q-DZH-V-4 are S. plebeian. V-HNYZ-bV-1 is probably a material of S. prattii or S. wardii promiscuous in V-HNYZ-V-1, which is very similar to W-SXXA-bV-1. W-SCHY-W-1 is S. roborowskii. |
| Key words： | molecular biology; cultivation of Danshen (Salvia miltiorrhiza); internal transcribed spacer (ITS); external transcribed spacer (ETS); molecular marker identification; genetic diversity |
| 发表期数： | 2023年9月第3期 |
| 引用格式： | 尹艳艳，任瑞花，冯洁，等. 新颖栽培丹参材料的rDNA内外转录间隔区（ITS和ETS）分子标记鉴定[J]. 中国科技论文在线精品论文，2023，16（3）：289-300. |
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