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小麦miR166基因家族鉴定及表达分析
发表时间：2024-03-29 浏览量：244 下载量：54
| 全部作者： | 雷晓彤，徐渴，孙若希，张树华，杨学举，赵勇 |
| 作者单位： | 河北农业大学农学院，华北作物改良与调控国家重点实验室，华北作物种质资源研究与利用教育部重点实验室 |
| 摘 要： | miR166作为重要的转录后调节因子，在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166（Tae-miR166）基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员，染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明，Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明，Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达，在籽粒和穗中表达量最高。荧光定量PCR结果表明，Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异，说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。 |
| 关 键 词： | 农艺学；Tae-miR166；小麦；进化特征；靶基因 |
| Title： | Genome-wide identification and expression analysis of miR166 gene family in whea |
| Author： | LEI Xiaotong, XU Ke, SUN Ruoxi, ZHANG Shuhua, YANG Xueju, ZHAO Yong |
| Organization： | North China Key Laboratory for Crop Germplasm Resources of Education Ministrys, State Key Laboratory of North China Crop, Improvement and Regulation, College of Agronomy, Hebei Agricultural University |
| Abstract： | As an essential post-transcriptional regulator, miR166 played an important role in plant growth and development and response to stress. The evolutionary characteristics and expression patterns of miR166 gene family in wheat (Tae-miR166) were analyzed by PmiREN, Ensembl Plants database, MEGA-X, DNAMAN software, RNAfold web server, WebLogo and psRNATarget online websites. Nineteen members of the Tae-miR166 gene family were searched in the PmiREN database, and chromosome localization revealed that Tae-miR166 were localized on 14 chromosomes. Sequence alignment revealed that the mature sequences of Tae-miR166 were highly conserved; evolutionary analysis showed that the Tae-miR166 gene family members were in 4 evolutionary branches, respectively. The target gene prediction results showed that the target genes of Tae-miR166 gene family included class III homologous heterotypic structural domain leucine zipper protein, β-galactosidase and calcium-dependent protein kinase. RNA-seq data analysis showed that 19 members of the Tae-miR166 family were expressed in 6 tissues of wheat, with the highest expression in grains and ears. The results of RT-qPCR showed that the expression patterns of Tae-miR166 family members were different after cadmium, drought and low temperature stress treatment, indicating that Tae-miR166 gene family played an important role in plant resistance to abiotic stress. This study provided a theoretical basis for further elucidating the function of miR166 gene family in wheat. |
| Key words： | agronomy; Tae-miR166; wheat; evolutionary characteristics; target gene |
| 发表期数： | 2024年3月第1期 |
| 引用格式： | 雷晓彤，徐渴，孙若希，等. 小麦miR166基因家族鉴定及表达分析[J]. 中国科技论文在线精品论文，2024，17（1）：41-49. |
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