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石仙桃多糖的提取及抗氧化活性的研究
发表时间：2008-04-30 浏览量：2271 下载量：894
| 全部作者： | 杨海花，翁寒梅，甘纯玑 |
| 作者单位： | 福建农林大学食品科学学院；福建农林大学生物技术中心 |
| 摘 要： | 本文首次在研究不同提取条件对多糖提取率影响的基础上，采用L9（34）正交试验优化石仙桃多糖的提取条件。得到石仙桃多糖的最佳提取条件为：浸提温度为80℃，浸提时间为5 h，料液比为1∶40。在此工艺条件下，石仙桃多糖的最佳提取率为2.25％。分别利用Fenton体系、邻苯三酚自氧化法产生·OH及·O2-，研究了石仙桃多糖的抗氧化活性。结果显示石仙桃多糖具有较好的抗氧化活性，抗氧化能力随浓度的增大而增强；粗多糖的清除自由基能力大于脱蛋白多糖，二者清除自由基的能力均大于或与VC相当。 |
| 关 键 词： | 食品科学；多糖；石仙桃；提取；抗氧化活性 |
| Title： | Extraction and antioxidant activities of polysaccharide from Pholidota chinensis Lindl |
| Author： | YANG Haihua, WENG Hanmei, GAN Chunji |
| Organization： | College of Food Science, Fujian Agriculture and Forestry University；Biotechnology Centre, Fujian Agriculture and Forestry University |
| Abstract： | The extraction of polysaccharide from Pholidota chinensis Lindl was studied by orthogonal experiment firstly. The best extraction condition of Pholidota chinensis Lindl polysaccharide was that: the time of extraction was 5 hours, the ratio of sample to water was 1∶40 and the temperature of extraction was 80℃. Under the best extraction conditions, the extraction efficiency of the polysaccharide of Pholidota chinensis Lindl was 2.25%. The effect on scavenging ·OH and ·O2- of Pholidota chinensis Lindl polysaccharide was studied by Fenton system and pyorgallol autoxidation method respectively. The results showed that: the Pholidota chinensis Lindl polysaccharide had a good anti-oxidantive ability; the ability increased along with the density; the crude polysaccharide�s ability was stronger than the purified polysaccharide�s, but their ability was all stronger than or equal to VC�s. |
| Key words： | food science; polysaccharide; Pholidota chinensis Lindl; extraction; antioxidant activities |
| 发表期数： | 2008年8月第8期 |
| 引用格式： | 杨海花，翁寒梅，甘纯玑. 石仙桃多糖的提取及抗氧化活性的研究[J]. 中国科技论文在线精品论文，2008，1（8）：927-930. |
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