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微生物的分子生物学鉴定方法

发表时间:2008-06-15  浏览量:2018  下载量:735
全部作者: 胡雪莲,张钧,单保庆
作者单位: 北京理工大学生命科学与技术学院;中国科学院生态研究中心环境水化学国家重点实验室
摘 要: 传统的微生物分类和鉴定方法主要是以微生物的形态学和生理生化等特性为依据,繁琐且费时。近年来,分子生物学的发展,为微生物的分类鉴定工作提供了简便准确的方法。分子生物学方法一部分用到聚合酶链式反应(polymerase chain reaction, PCR)扩增,比如:变性和温度梯度凝胶电泳、单链构建多态性、限制性片段长度多态性、自动核糖体间隔基因分析等。一些不依赖PCR的方法也得到了广泛的应用,如荧光原位杂交、DNA联合分析等。本文重点综述了几种广泛应用的分子生物学技术,并对其原理及优缺点进行了阐述。
关 键 词: 微生物学;分类和鉴定;综述;分子生物学方法;聚合酶链式反应
Title: The molecular methods for identification of hydrophytic microbes
Author: HU Xuelian, ZHANG Jun, SHAN Baoqing
Organization: School of Life Science and Technology, Beijing Institute of Technology;Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences
Abstract: The classification and identification of the microorganisms is an important way to the study of microorganism. Traditionally, it is tedious and time-consuming because it follows the character of bioecology .With the development of molecular biology, the way of microbic identification becomes more precise. Most of the molecular biology methods are involved in polymerase chain reaction (PCR), for instants, temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis, random sequencing in clone libraries, single-strand conformation polymorphism, terminal-restriction fragment length polymorphism, and so on. Some methods which are not involved in PCR are also be used widely, such as fluorescence in-situ hybridization, DNA re-association analysis. On the whole, we can get different information from different methods. This paper introduces some useful methods and shows their advantages and disadvantages.
Key words: microbiology; classification and identification; review; molecular biological methods; polymerase chain reaction
发表期数: 2008年10月第11期
引用格式: 胡雪莲,张钧,单保庆. 微生物的分子生物学鉴定方法[J]. 中国科技论文在线精品论文,2008,1(11):1213-1217.
 
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