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转LOS5/ABA3基因早熟禾(Poa pratensis L.)的获得和抗旱及抗寒性分析

发表时间:2008-06-15  浏览量:1702  下载量:547
全部作者: 宋哲,章家长,楮庆全,李召虎
作者单位: 中国农业大学农学与生物技术学院
摘 要: 本文以早熟禾品种‘优异’成熟种胚诱导的愈伤为外植体,通过农杆菌介导法导入LOS5/ABA3基因,在含有100 mg/L潮霉素的改良MS培养基上筛选、分化出完整植株。经PCR检测,表明得到35株整合了LOS5/ABA3基因的阳性植株。转基因植株的内源脱落酸(ABA)含量在正常环境及干旱胁迫下均显著高于野生型,其叶片离体失水速率和干旱胁迫后的叶片相对电导率也均显著低于后者。转LOS5/ABA3基因植株在干旱胁迫后的萎蔫叶片数占总叶片数的百分比显著低于野生型,表明早熟禾的转LOS5/ABA3基因植株与野生型相比具有更强的抗旱能力。冷胁迫后,野生型植株轻微萎蔫、生长速率降低,而转基因植株生长正常,抗寒性明显增强。
关 键 词: 分子遗传学;干旱胁迫;农杆菌;早熟禾;低温胁迫;LOS5基因
Title: Acquisition of LOS5/ABA3 transgenic Kentucky bluegrass(Poa pratensis L.) and characteristics under drought and cold stress
Author: SONG Zhe, ZHANG Jiachang, CHU Qingquan, LI Zhaohu
Organization: College of Agronomy and Biotechnology, China Agricultural University
Abstract: Agrobacterium-mediated transformation was used on callus induced from the mature ‘merit’ Kentucky bluegrass seeds as explants. Callus containing LOS5/ABA3 gene were transferred to the improved MS-medium containing 100 mg/L Hygromycin B and callus that carried exposed to Hygromycin B, were regenerate as plants. The LOS5/ABA3 gene were transmitted to 35 plants which were confirmed by PCR analysis. Physiological analysis showed that the ABA content in LOS5/ABA3 transgenic plants were higher than in wild-type plants, both under normal and drought stress conditions. The rate of water lose from Kentucky bluegrass removed from the plant was lower in LOS5/ABA3 transgenic plants than in wild-type plants. Relative conductances under drought stress were lower in LOS5/ABA3 transgenic plants than in the wild-type plants. The percentage of leaves that faded under drought stress in drought stress examination, the LOS5/ABA3 transgenic plant’ the percentage of faded leaves in all leaves is lower then in the wild type plants, which proved that LOS5/ABA3 transgenic plants enhanced ability to tolerate drought stress. Under cold stress, wild-type plants had some leaves fading and their growth rate decreased, while LOS5/ABA3 transgenic plants grew normally, which proved that LOS5/ABA3 transgenic plants have greater ability to tolerate cold stress compare wild-type plants.
Key words: molecular genetics; drought stress; Agrobacterium; Kentucky bluegrass; low temperature stress; LOS5
发表期数: 2008年10月第11期
引用格式: 宋哲,章家长,楮庆全,等. 转LOS5/ABA3基因早熟禾(Poa pratensis L.)的获得和抗旱及抗寒性分析[J]. 中国科技论文在线精品论文,2008,1(11):1233-1240.
 
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