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脂肪酶产生菌的分离筛选及产酶条件研究

发表时间:2008-06-15  浏览量:1852  下载量:686
全部作者: 周海霞,袁丽红,欧阳平凯
作者单位: 南京工业大学制药与生命科学学院
摘 要: 本文以麻疯树油为唯一碳源,从以粉碎的麻疯树种子处理过的土壤中分离筛选出1株脂肪酶活性较高的菌株,初步鉴定为假单胞菌属(Pseudomonas)。实验考察了碳源、氮源、无机盐及发酵工艺对产酶的影响。摇瓶发酵结果表明,该菌株最适产酶培养基的组成是(%,v/v或w/v):橄榄油2;酵母膏0.5;(NH4)2SO4 0.5;MgCl2·6H2O 0.5;最适产酶温度为30℃;最佳产酶pH为6.5;转速180 r/min;发酵培养36 h酶活达到最高,为14.17 U/mL.
关 键 词: 发酵工程;脂肪酶;假单胞菌;分离筛选;产酶条件
Title: Screening of lipase-producing strain and optimization of fermentation conditions
Author: ZHOU Haixia, YUAN Lihong, OUYANG Pingkai
Organization: College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology
Abstract: A wild strain of lipase-producing bacteria was isolated from the soil pretreated with Jatropha curcas seed powder by using Jatropha oil as sole carbon source and identified as Pseudomonas sp.. The fermentation conditions of the lipase-production strain were optimized by studying effects of carbon sources, nitrogen sources, and fermentation technics. The results showed that the optimal medium composition for lipase producing was as follows(%,v/v or w/v ): olive oil 2.0, yeast extract 0.5, (NH4)2SO4 0.5, MgCl2·6H2O 0.5. The optimal initial pH of fermentation medium was 6.5. The maximum yield of 14.17 U/mL was obtained when Pseudomonas sp. was shake-cultivated at 30℃ for 36 h with 180 r/min.
Key words: fermentation engineering; lipase; Pseudomonas sp.; screening; fermentation conditions
发表期数: 2008年10月第11期
引用格式: 周海霞,袁丽红,欧阳平凯. 脂肪酶产生菌的分离筛选及产酶条件研究[J]. 中国科技论文在线精品论文,2008,1(11):1246-1252.
 
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