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小鼠乳腺中Heregulin-α的信号转导通路

发表时间:2008-06-15  浏览量:1508  下载量:512
全部作者: 耿丽晶,李庆章
作者单位: 乳品科学教育部重点实验室,东北农业大学生命科学学院
摘 要: 为研究小鼠乳腺发育、泌乳及退化过程中调控因子Heregulin-α(HRG-α)的信号转导通路,分别取妊娠9 d、泌乳12 d以及退化6 d的小鼠乳腺组织进行体外培养,对照组培养基为无血清培养基,处理组培养基中则只添加HRG-α.培养24 h后分别提取乳腺上皮细胞总蛋白,用免疫印迹法检测对照组和处理组乳腺上皮细胞中信号分子STAT3,STAT5,p44/42 MAPK和p38 MAPK的磷酸化情况,并通过ELISA方法检测对照组和处理组乳腺上皮细胞中信号分子PKC的磷酸化情况。结果表明,HRG-α能够促进妊娠期小鼠乳腺上皮细胞p44/42 MAPK,p38 MAPK和PKC的磷酸化;HRG-α能够促进泌乳期小鼠乳腺上皮细胞STAT5和p38 MAPK的磷酸化,而同时又抑制PKC的磷酸化;HRG-α能够增强退化期小鼠乳腺上皮细胞STAT3的磷酸化,并抑制PKC的磷酸化。
关 键 词: 生物化学与分子生物学;信号转导;蛋白质印迹;Heregulin-α;小鼠;乳腺
Title: Study on signal transduction pathway of Heregulin-α in mammary gland of mouse
Author: GENG lijing, LI Qingzhang
Organization: Key Laboratory of Dairy Science, Ministry of Education, Couege of Life Sciences, Northeast Agricultural University
Abstract: To determine the signal transduction pathway of Heregulin-α(HRG-α) in mammary gland, the mammary gland of mouse at 9 days in pregnancy, 12 days in lactation and 6 days in involution were cultured in vitro. Each individual mammary gland was cultured in the media Waymouth’s MB752/1 with or without HRG-α. Following 24 hours of culture, each mammary gland was digested to obtain epithelial cells, which were lysed and the lysate was used for Western blot analysis. The phosphory lation of PKC was detected by ELISA. Western blot results showed that HRG-α enhanced the phosphorylation of p44/42 MAPK, p38 MAPK and PKC in pregnancy; and, HRG-α promoted the phosphorylation of STAT5 and p38 MAPK, while it inhibited PKC phosphorylation in lactation; in involution, HRG-α induced STAT3 phosphorylation and inhibited PKC phosphorylation.
Key words: biochemistry and molecular biology; signal transduction; Western blot; Heregulin-α; mouse; mammary gland
发表期数: 2008年10月第11期
引用格式: 耿丽晶,李庆章. 小鼠乳腺中Heregulin-α的信号转导通路[J]. 中国科技论文在线精品论文,2008,1(11):1260-1265.
 
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