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中华花鳅外周血细胞形态的观察

发表时间:2008-07-15  浏览量:1895  下载量:660
全部作者: 高泽霞,王卫民
作者单位: 华中农业大学水产学院,华中农业大学农业动物遗传育种与繁殖教育部重点实验室
摘 要: 本文报道了中华花鳅外周血细胞的显微结构,应用常规血涂片Wright-Giemsa(WG)染液联合染色,在油镜下观察中华花鳅的外周血细胞。中华花鳅的外周血中可区分出下列各种类型的血细胞:红细胞、嗜中性粒细胞、嗜酸性粒细胞、单核细胞、淋巴细胞和血栓细胞,还可见到幼红细胞、正在分裂的红细胞和正在分裂的血栓细胞。白细胞中血栓细胞和嗜中性粒细胞较多;嗜酸性粒细胞体积最大,血栓细胞体积最小。文章还分别对各类血细胞的形态结构进行了描述,结果表明:中华花鳅外周血细胞的形态特征与其他鱼类基本相似,为该鱼今后的人工繁殖驯养、疾病诊断治疗等工作提供参考依据。
关 键 词: 水产学基础学科;中华花鳅;血细胞;显微结构
Title: Observation on the peripheral blood cells morphology of Chinese spined loach, Cobitis sinensis
Author: GAO Zexia, WANG Weimin
Organization: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education,Huazhong Agricultural University
Abstract: The morphology of the peripheral blood cells of Chinese spined loach (Cobitis sinensis) has been studied by light microscopy. Blood samples were collected from 10 fish by caudal vein, smeared and stained with Wright-Giemsa (WG) fluid. Observation of the peripheral blood cells using the microphotography showed that there were six types of cells including erythrocyte, neutrophil granulocyte, eosinophil granulocyte, monocyte, lymphocyte and thrombocyte. The immature erythrocytes were presented in peripheral blood. Amitosis was observed in both erythrocyte and thrombocyte. The thrombocyte and neutrophil granulocyte were abundant in leucocyte, while eosinophil granulocyte was the biggest, and thrombocyte was the smallest. The microstructure of each type of peripheral blood cell was described. The results indicated that the characteristics of the peripheral blood cells in Cobitis sinensis were basically similar to other fishes.
Key words: basic disciplines of fisheries; Cobitis sinensis; bloods cells; microstructure
发表期数: 2008年11月第13期
引用格式: 高泽霞,王卫民. 中华花鳅外周血细胞形态的观察[J]. 中国科技论文在线精品论文,2008,1(13):1517-1522.
 
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