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复方银黄微型灌肠剂对大鼠腹腔巨噬细胞NO及iNOS的影响

发表时间:2008-08-15  浏览量:1412  下载量:578
全部作者: 黄磊松,杨斌,廖润玲,蔡文娥,刘华钢
作者单位: 广西南宁市中医院检验科;广西医科大学药学院
摘 要: 研究复方银黄微型灌肠剂(Yinhuang micro-enema compound,YHMCE)对巨噬细胞释放一氧化氮(nitric oxide, NO)及诱导型一氧化氮合酶(induced nitricoxide synthase, iNOS)活性的影响,探讨其抗炎作用分子机理。体外培养大鼠腹腔巨噬细胞(peritoneal macrophage,PMФ),采用氧化还原酶法、免疫细胞化学法和RT-PCR分别测定不同剂量YHMCE对内毒素(lipopolysaccharide, LPS)诱导大鼠腹腔巨噬细胞NO生成量、iNOS活性及iNOS mRNA表达水平的影响。结果表明:YHMCE能降低大鼠腹腔巨噬细胞iNOS的活性及iNOS mRNA的表达水平,减少LPS诱导的NO大量产生和释放,并呈作用剂量依赖关系。YHMCE对大鼠腹腔巨噬细胞NO产生的调节可发生在iNOS的转录水平,抑制iNOS从头合成,减少NO合成与释放,可能是其发挥抗炎作用的分子作用机理之一。
关 键 词: 中药药理;腹腔巨噬细胞;复方银黄微型灌肠剂;一氧化氮;诱导型一氧化氮合酶
Title: Effect of Yinhuang micro-enema compound on NO and induced nitricoxide synthase in rat peritoneal macrophage
Author: HUANG Leisong, YANG Bin, LIAO Runling, CAI Wen’e, LIU Huagang
Organization: Department of Clinical Laboratory of Guangxi Nanning Hospital of Traditional Chinese Medicine;School of Pharmaceutical Sciences, Guangxi Medical University
Abstract: To study the effect of Yinhuang micro-enema compound (YHMCE) on release of nitric oxide (NO) and the activity of induced nitric oxide synthase (iNOS) so as to elucidate its anti-inflammatory mechanisms. Peritoneal macrophage (PMФ) obtained from normal rats were cultivated in vitro. The production of NO, the activity and mRNA expression of iNOS in peritoneal macrophages of mice administered with different dose of YHMCE were observed by nitrate reductase method, immunocytochemical method and RT-PCR, respectively. YHMCE can inhibit the activity and mRNA expression of iNOS with dose-dependence in rat peritoneal macrophages, and reduce the generation and release of NO. The regulation of YHMCE on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS, inhibiting the iNOS’s denovo synthesis and reducing the generation and release of NO. This may be one of its anti-inflammatory mechanisms.
Key words: herbal pharmacology; peritoneal macrophage; Yinhuang micro-enema compound; nitric oxide (NO); induced nitric oxide synthace (iNOS)
发表期数: 2008年12月第15期
引用格式: 黄磊松,杨斌,廖润玲,等. 复方银黄微型灌肠剂对大鼠腹腔巨噬细胞NO及iNOS的影响[J]. 中国科技论文在线精品论文,2008,1(15):1695-1699.
 
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