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醋柳黄酮中熊果酸的提取分离与含量测定

发表时间:2008-08-15  浏览量:1446  下载量:657
全部作者: 谭承佳,马家骅,王毓杰,张艺
作者单位: 绵阳师范学院生命科学与技术学院;成都中医药大学药学院
摘 要: 目的:从醋柳黄酮中提取分离熊果酸并建立其含量测定方法。方法:采用硅胶柱层析法从醋柳黄酮氯仿浸提物中分离得到化合物,经氢核磁共振(1H-NMR),碳核磁共振(13C-NMR)鉴定为熊果酸;采用DiamonsilTM(钻石)C18(250 mm×4�6 mm,5 μm),甲醇-8 mmol·L-1四丁基溴化铵溶液-三乙胺(90∶10∶0�2)为流动相,流速1 mL·min-1,紫外检测波长210 nm,柱温40℃.结果:熊果酸的标准曲线线性范围为0�59~8�85 μg(r=0�999 6,n=5);平均加样回收率为97�11%,相对标准差(RSD)为1�20%(n=6)。结论:该法简便、准确、重复性好,适用于醋柳黄酮中熊果酸的定量分析。
关 键 词: 药物化学;醋柳黄酮;熊果酸;高效液相色谱
Title: Extraction and determination of ursolic acid from sea-buckthorn flavonoid
Author: TAN Chengjia, MA Jiahua, WANG Yujie, ZHANG Yi
Organization: College of Life Science & Biotechnology, Mianyang Normal University;College of Traditional Chinese Pharmacy, Chengdu University of Traditional Chinese Medicine
Abstract: Objective: To extract ursolic acid from sea-buckthorn flavonoid and determine the content by HPLC. Methods: The compound was obtained from the chloroform extracted fraction of sea-buckthorn flavonoid by column chromatograph. Its structure was identified by 1H-NMR, 13C-NMR as ursolic acid; DiamonsilTM C18(250 mm×4.6 mm, 5 μm) was employed, the mobile phase consisted of methanol -8mmol·L-1 tetrabutyl ammonium bromide-triethylamine (90∶10∶0.2) with a flow velocity of 1.0 mL/min. The detective wavelength was set at 210 nm and the column temperature was at 40℃. Results: The calibration curve was linear in the range of 0.59~8.85 μg (r=0.999 6, n=5), and the average recovery was 97.11%, RSD was 1.20% (n=6). Conclusion: The method is simple and accurate in determining the content of ursolic acid in sea-buckthorn flavonoid.
Key words: pharmaceutical chemistry; sea-buckthorn flavonoid; ursolic acid; high performance liquid chromatography (HPLC)
发表期数: 2008年12月第15期
引用格式: 谭承佳,马家骅,王毓杰,等. 醋柳黄酮中熊果酸的提取分离与含量测定[J]. 中国科技论文在线精品论文,2008,1(15):1700-1703.
 
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