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一株絮凝剂产生菌X3的筛选及所产絮凝剂PX的性质研究

发表时间:2008-08-31  浏览量:1450  下载量:673
全部作者: 陈若维,雷恒毅,黎忠
作者单位: 中山大学环境工程系
摘 要: 通过稀释涂板法与划线分离法从土壤中筛选出4株产絮凝剂细菌,选择其中的一株高效产絮凝剂菌X3做进一步研究,其培养48 h后的发酵液在Ca2+离子的协同作用下对高岭土悬浊液表现出良好的絮凝活性(90%以上)。X3的形态学特征和生理学特征与芽胞杆菌(Bacillus sp.)一致,因此菌株X3被鉴定为Bacillus sp.X3在发酵培养基中培养48 h后絮凝活性达到最高点,利用SALEHIZADEH提出的方法提取发酵液中的絮凝活性产物,命名为PX.絮凝活性产物PX的产量是0.4 g/L,主要成分为58.4%多糖与1.17%蛋白质。PX具有良好的pH稳定性与温度稳定性。菌种发酵液在常温密封下存放数天而絮凝活性只有轻微下降,因此PX具有良好的应用前景。
关 键 词: 环境科学技术基础学科;微生物絮凝剂;稀释涂板与划线分离;芽孢杆菌;热稳定性
Title: Screening of a bioflocculant producing bacterium X3 and characteristic study of its bioflocculant product PX
Author: CHEN Ruowei, LEI Hengyi, LI Zhong
Organization: Department of Environmental Science and Engineering, Sun Yat-sen University
Abstract: Four strains of flocculant-producing bacteria were isolated from the soil sample collected inside Sun Yat-sen University, and X3, one of the four, was selected for further study due to its high flocculating activity and rapid growth. The flocculating activity reached its highest after 48 h cultivation, when the bacteria were in the stationary phase. After the bacteria entered the decline phase the flocculating activity decreased significantly, from which we can infer that the bioflocculant produced by X3 can be biodegraded and is environmentally friendly. According to the morphological and physiological characteristics of X3, the strain was identified as Bacillus sp. About 0.95 g of crude bioflocculant product PX was recovered from 250 mL of culture broth through the process reported by SALEHIZADEH, and its carbohydrate and protein content was measured using the phenol-sulfuric acid method and Bradford method, respectively. The main composition of PX was 58.4% of polysaccharide and 1.17% of protein. PX had a fine pH and thermal stability and the culture broth which was stored in a sealed beaker flask at normal temperature still kept a high flocculating activity after six days or more. Thus the bioflocculant PX is expected to be a suitable alternative to inorganic and synthetic flocculants.
Key words: basic disciplines of environmental science and technology; bioflocculant; loop dilution & streak plate; Bacillus sp.; thermal stability
发表期数: 2008年12月第16期
引用格式: 陈若维,雷恒毅,黎忠. 一株絮凝剂产生菌X3的筛选及所产絮凝剂PX的性质研究[J]. 中国科技论文在线精品论文,2008,1(16):1768-1773.
 
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