您的位置:首页  > 论文页面

高效液相色谱法测定叶菜中土霉素的残留量

发表时间:2008-08-31  浏览量:1442  下载量:665
全部作者: 崔馨,乔显亮,王震,韩成伟
作者单位: 大连理工大学工业生态与环境工程教育部重点实验室
摘 要: 通过Na2EDTA-McIlvaine缓冲液(pH4)超声振荡提取样品的前处理方法,以及优化的高效液相色谱(high performance liquid chromatography, HPLC)条件,建立了3种代表性叶菜(生菜、芹菜和小白菜)中土霉素的定量检测方法。选用的色谱柱为Hypersil BDS C18(250 mm×4.6 mm i.d., 5 μm)反相柱;流动相流速为1.5 mL/min;柱温35℃;检测器为二极管阵列检测器;检测波长为355 nm.由于3种叶菜组织成分存在差异,需采用不同的流动相比例对这3种叶菜中的土霉素进行分离检测。生菜、芹菜和小白菜样品选用的流动相(甲醇∶乙腈∶1%甲酸,pH3.0)体积比分别为10∶15∶75,10∶10∶80,6∶6∶88. 这3种叶菜中土霉素的加标回收率为66.8%~82.9%,相对标准偏差小于10%.该方法具有回收率高和重现性好等优点,可用于叶菜中土霉素残留的检测。
关 键 词: 环境科学技术其他学科;土霉素;叶菜;高效液相色谱;提取
Title: Detection of oxytetracycline residues in leaf-vegetables by high performance liquid chromatography
Author: CUI Xin, QIAO Xianliang, WANG Zhen, HAN Chengwei
Organization: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, Dalian University of Technology
Abstract: The residues of oxytetracycline in lettuce, celery and pakchoi cabbage were measured with optimum procedure of high performance liquid chromatography (HPLC). Oxytetracycline in the three leaf-vegetable samples was extracted thrice by Na2EDTA-McIlvaine buffer (pH4). The filtrate was examined by HPLC with a reversed phase column Hypersil BDS C18 (250 mm×4.6 mm i.d., 5 μm). The flowing rate of mobile phase was 1.5 mL/min, and the temperature of column was 35℃. The wavelength of DAD detector was 355 nm. Because the three leaf-vegetables are of different compositions, the mobile phase was adjusted to optimum ratio to get satisfactory resolution. The ratios of mobile phase (methanol∶acetonitrile∶1% formic acid, pH3.0) were 10∶15∶75, 10∶10∶80, 6∶6∶88 for lettuce, celery and pakchoi cabbage, respectively. The recovery of oxytetracycline was from 66.8% to 82.9%, and the RSD values of replicates were less than 10%. The results indicated that the method is accurate and stable, which can be used to detect oxytetracycline residues in leaf-vegetables. This analysis procedure will be useful in the study of the uptake of oxytetracycline by vegetables and in the risk assessment of food contamination.
Key words: environmental science and technology to other subjects; oxytetracycline; leaf-vegetable; high performance liquid chromatography (HPLC); extraction
发表期数: 2008年12月第16期
引用格式: 崔馨,乔显亮,王震,等. 高效液相色谱法测定叶菜中土霉素的残留量[J]. 中国科技论文在线精品论文,2008,1(16):1778-1783.
 
0 评论数 0
暂无评论
友情链接