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一株纤维素降解真菌的筛选及其降解条件的初步研究
发表时间：2009-04-15 浏览量：1886 下载量：610
| 全部作者： | 张瑞永，夏金兰，张倩，彭娟花，张成桂，熊晶，武顺，金德才 |
| 作者单位： | 中南大学生物冶金教育部重点实验室，资源加工与生物工程学院 |
| 摘 要： | 从长沙宁乡灰汤温泉附近的水样中分离获得1株具有较强纤维素降解能力的菌株，命名为NX1-1，该菌株最适生长温度为40℃，最适生长pH5�0～pH8�0�菌株形态学以及ITS rDNA和28 S rDNA D1/D2序列分析的鉴定结果表明：该菌株属于Aspergillus fumigatus种。对该菌株产酶特性进行了初步研究，最适产酶条件为：氮源(NH4)2SO4，接种量10%，40℃，初始pH6�0，培养60~96 h� |
| 关 键 词： | 微生物学；分离鉴定；系统发育分析；烟曲霉；纤维素酶；产酶条件 |
| Title： | Screening and characterization of cellulose-producing Aspergillus fumigatus NX1-1 |
| Author： | ZHANG Ruiyong, XIA Jinlan, ZHANG Qian, PENG Juanhua, ZHANG Chenggui, XIONG Jing, WU Shun, JIN Decai |
| Organization： | Key Laboratory of Biometallurgy, Ministry of Education, School of Minerals Processing and Bioengineering, Central South University |
| Abstract： | A cellulose-producing strain named NX1-1 was isolated from environmental samples collected from hot springs of Ningxiang County, Hunan Province, China. The optimum temperature for growth was 40℃ and the optimum pH was 5.0～8.0. The cell shape suggested that strain NX1-1 was likely a strain of Aspergillus fumigatus. Furthermore, a phylogenetic analysis based on ITS rDNA and 28 S rDNA D1/D2 sequences showed that the strain NX1-1 should be classified into the species Aspergillus fumigatus. The most suitable conditions for the cellulose production were: inorganic nitrogen source (NH4)2SO4, 10% of inoculation, 40℃, initial pH6.0, culturing for 60～96 h. |
| Key words： | isolation and identification; phylogenetic analysis; Aspergillus fumigatus; cellulose; fementation condition |
| 发表期数： | 2009年4月第7期 |
| 引用格式： | 张瑞永，夏金兰，张倩，等. 一株纤维素降解真菌的筛选及其降解条件的初步研究[J]. 中国科技论文在线精品论文，2009，2（7）：674-681. |
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