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一株纤维素降解真菌的筛选及其降解条件的初步研究

发表时间:2009-04-15  浏览量:1530  下载量:526
全部作者: 张瑞永,夏金兰,张倩,彭娟花,张成桂,熊晶,武顺,金德才
作者单位: 中南大学生物冶金教育部重点实验室,资源加工与生物工程学院
摘 要: 从长沙宁乡灰汤温泉附近的水样中分离获得1株具有较强纤维素降解能力的菌株,命名为NX1-1,该菌株最适生长温度为40℃,最适生长pH5�0~pH8�0�菌株形态学以及ITS rDNA和28 S rDNA D1/D2序列分析的鉴定结果表明:该菌株属于Aspergillus fumigatus种。对该菌株产酶特性进行了初步研究,最适产酶条件为:氮源(NH4)2SO4,接种量10%,40℃,初始pH6�0,培养60~96 h�
关 键 词: 微生物学;分离鉴定;系统发育分析;烟曲霉;纤维素酶;产酶条件
Title: Screening and characterization of cellulose-producing Aspergillus fumigatus NX1-1
Author: ZHANG Ruiyong, XIA Jinlan, ZHANG Qian, PENG Juanhua, ZHANG Chenggui, XIONG Jing, WU Shun, JIN Decai
Organization: Key Laboratory of Biometallurgy, Ministry of Education, School of Minerals Processing and Bioengineering, Central South University
Abstract: A cellulose-producing strain named NX1-1 was isolated from environmental samples collected from hot springs of Ningxiang County, Hunan Province, China. The optimum temperature for growth was 40℃ and the optimum pH was 5.0~8.0. The cell shape suggested that strain NX1-1 was likely a strain of Aspergillus fumigatus. Furthermore, a phylogenetic analysis based on ITS rDNA and 28 S rDNA D1/D2 sequences showed that the strain NX1-1 should be classified into the species Aspergillus fumigatus. The most suitable conditions for the cellulose production were: inorganic nitrogen source (NH4)2SO4, 10% of inoculation, 40℃, initial pH6.0, culturing for 60~96 h.
Key words: isolation and identification; phylogenetic analysis; Aspergillus fumigatus; cellulose; fementation condition
发表期数: 2009年4月第7期
引用格式: 张瑞永,夏金兰,张倩,等. 一株纤维素降解真菌的筛选及其降解条件的初步研究[J]. 中国科技论文在线精品论文,2009,2(7):674-681.
 
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