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稻瘟病菌一个假定的GPCR基因功能分析

发表时间:2009-04-15  浏览量:1711  下载量:793
全部作者: 方坤海,易运萍,郑文辉,陈新,刘文德,鲁国东,许金荣,王宗华
作者单位: 福建农林大学功能基因组学研究中心;普渡大学
摘 要: 对稻瘟病菌基因组中一个假定的GPCR基因Mgg_05072.5进行了生物信息学和分子遗传学初步分析。结果表明:该GPCR基因所编码的蛋白具有7个跨膜结构域,可能定位在细胞膜上。经基因敲除表明:敲除突变体的产孢量比野生型的显著增加,但是其生长速度、致病性等与野生的无明显差异。结果有助于进一步了解GPCRs在稻瘟病菌生长发育、侵染致病过程中的作用。
关 键 词: 微生物遗传学;稻瘟病菌;G蛋白偶联受体;基因敲除;生物信息学
Title: Functional analysis of a putative G-protein coupled receptor in Magnaporthe grisea
Author: FANG Kunhai, YI Yunping, ZHENG Wenhui, CHEN Xin, LIU Wende, LU Guodong, XU Jinrong, WANG Zonghua
Organization: The Functional Genomics Center, Fujian Agriculture and Forestry University; Purdue University
Abstract: This study applied bioinformatics and molecular genetics approaches to study the function of a putative GPCR gene Mgg_05072.5 in Magnaporthe grisea. Bioinformatics analysis showed that the gene encodes a protein with seven transmembrance domains. A constant increase of conidiation was observed in gene knock-out mutant ΔMgg_05072-2 when compared to wild-type strain. However, the mutant was not affected in growth rate and pathogenicity. The results will facilitate further characterization of GPCR function in the fungal growth, development and pathogenesis.
Key words: microbial genetics; Magnaporthe grisea; G-protein coupled receptors; gene knock-out; bioinformatics
发表期数: 2009年4月第7期
引用格式: 方坤海,易运萍,郑文辉,等. 稻瘟病菌一个假定的GPCR基因功能分析[J]. 中国科技论文在线精品论文,2009,2(7):682-686.
 
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