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高效液相色谱法测定藏药多血康中没食子酸的含量

发表时间:2009-06-15  浏览量:1551  下载量:562
全部作者: 郑海杰,耿志鹏,冯冠榕,王舒,张艺,赖先荣,索朗其美
作者单位: 成都中医药大学民族医药学院;西藏藏医学院
摘 要: 目的:建立常用藏药多血康中没食子酸含量测定方法。方法:采用高效液相色谱法(high performance liquid chromatography,HPLC)测定多血康中没食子酸的含量。结果:没食子酸浓度在9.6~240 μg/L范围内,与峰面积积分值线性关系良好。该方法的平均回收率为99.50%,相对标准偏差(relative standard deviation,RSD)为1.38%(n=6)。结论:高效液相色谱法准确、简便、快速,适合于该制剂的质量控制。
关 键 词: 民族医学;多血康;没食子酸;高效液相色谱法;含量测定
Title: Content determination of gallic acid in Duoxuekang by HPLC
Author: ZHENG Haijie, GENG Zhipeng, FENG Guanrong, WANG Shu, ZHANG Yi, LAI Xianrong, SUOLANGQIMEI
Organization: National Medical College, Chengdu University of Traditional Chinese Medicine;Tibetan Traditional Medical College
Abstract: Objective: To establish a content determination method of gallic acid in Duoxuekang. Methods: The content of gallic acid in Duoxuekang was determined by high performance liquid chromatography (HPLC). Results: The gallic acid concentration had good linear relation with peak area integral quantity in the range of 9.6~240 mg/L, the average recovery rate was 99.50%, and relative standard deviation(RSD) was 1.38% (n=6). Conclusion: The method(HPLC) has high accuracy, reliability and reproducibility, so it can be used in the quality control of the preparation.
Key words: Ethnic medicine; Duoxuekang; gallic acid; high performance liquid chromatography; content determination
发表期数: 2009年6月第11期
引用格式: 郑海杰,耿志鹏,冯冠榕,等. 高效液相色谱法测定藏药多血康中没食子酸的含量[J]. 中国科技论文在线精品论文,2009,2(11):1163-1166.
 
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