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玉米地上部膜结合NFR分离及酶学性质初步探索

发表时间:2009-08-31  浏览量:1485  下载量:670
全部作者: 李文学,杨荣新,张福锁
作者单位: 中国农业大学资源与环境学院,教育部植物-土壤相互作用重点实验室
摘 要: 以玉米为材料,通过DEAE-纤维素离子交换层析、SP-sepharose FF离子交换层析等一系列分离纯化手段,对玉米地上部膜结合、依赖还原型辅酶Ⅰ(NADH)/还原型辅酶Ⅱ(NADPH)的铁还原酶[NAD(P) H-dependent ferric reductase, NFR]的酶学性质进行了初步探索。实验表明:玉米地上部膜结合NFR对于电子供体NADPH较电子供体NADH有更小的Km值,最适催化反应温度为30~40℃,最适反应pH值为6.0,而且该酶对温度极为敏感,在大于40℃下保温15 min,酶活性即显著下降。
关 键 词: 植物营养学;玉米;膜结合铁还原酶;DEAE-纤维素离子交换层析;SP-sepharose FF离子交换层析
Title: Preliminary research on purification and characterization of membrane binding NAD(P) H-dependent ferric reductase from maize shoots
Author: LI Wenxue, YANG Rongxin, ZHANG Fusuo
Organization: Key Laboratory of Plant-Soil Interactions, Ministry of Education, College of Resources and Environmental Sciences, China Agricultural University
Abstract: In this experiment, the characteristics of the membrane binding ferric reductase in maize shoots are primarily explored by DEAE-cellulose anion exchange chromatography and SP-sepharose FF cation exchange chromatography. Results show that the optimum catalyzing pH is 6.0, with optimum temperature in the range between 30~40℃, and the enzyme is extraordinarily sensitive to temperature, the enzyme activity significantly decreases when it is kept exceeding 40℃ for 15 min. These characterizations are obviously different from soluble ferric reductase.
Key words: plant nutrition; maize; membrane binding ferric reductase; DEAE-cellulose anion exchange chromatography; SP-sepharose FF cation exchange chromatography
发表期数: 2009年8月第16期
引用格式: 李文学,杨荣新,张福锁. 玉米地上部膜结合NFR分离及酶学性质初步探索[J]. 中国科技论文在线精品论文,2009,2(16):1628-1632.
 
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