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利用水松实生苗茎段诱导植株再生
发表时间：2009-08-31 浏览量：2647 下载量：776
| 全部作者： | 李博，李火根，王光萍 |
| 作者单位： | 南京林业大学林木遗传与生物技术省部共建教育部重点实验室；桂林高等师范专科学校化学与工程技术系 |
| 摘 要： | 以水松种子无菌萌发60 d的实生苗茎段为外植体，通过比较培养基种类、激素水平、蔗糖浓度对腋芽诱导、伸长及生根诱导的影响，建立水松实生苗茎段诱导植株再生体系。水松组织培养及植株再生的较适培养条件为：1） 诱导腋芽培养基，MS+0.1 mg/L ZT+维生素C（VC）5.0 mg/L+蔗糖3%；2） 腋芽伸长培养基，MS+VC 5.0 mg/L+蔗糖3%；3） 生根培养基，1/2MS+NAA 0.5 mg/L+VC 5.0 mg/L+蔗糖1%. 伸长的腋芽在添加了0.5 mg/L NAA的1/2MS培养基中培养2个月后，生根率达58.8%. 在草炭∶珍珠岩=7∶3 的基质上，试管苗的移栽成活率为70%. |
| 关 键 词： | 森林培育学；水松；茎段；组织培养；器官发生 |
| Title： | Plantlet regeneration of Glyptostrobus pensilis (Lamb.) K. Koch from seedling stem |
| Author： | LI Bo, LI Huogen, WANG Guangping |
| Organization： | Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University；Department of Chemistry & Engineering Technlolgy, Guilin Normal College |
| Abstract： | This paper reported the plantlet regeneration of Glyptostrobus pensilis through organogenesis using stems excised from 60-day-old in vitro seedlings as explants. The optimized protocol for plantlet regeneration of Glyptostrobus pensilis was developed by comparing the effects of media, hormone level, and sucrose concentration on axillary bud induction, elongation, and shoot rooting. The suitable media for axillary bud induction, elongation and shoot rooting were MS+0.1 mg/L Zeatin (ZT)+Vitamin C 5.0 mg/L+sucrose 3%, MS+Vitamin C 5.0 mg/L+sucrose 3%, 1/2MS+NAA 0.5 mg/L+Vitamin C 5.0 mg/L+sucrose 1% respectively. The elongated axillary buds were rooted at a rate of 58.8% on half-strength MS medium with 0.5 mg/L α-naphthaleneacetic acid (NAA) in 2 months. Rooted plantlets were successfully transplanted in substrate (peat∶perlite =7∶3) with a 70% survival rate. |
| Key words： | silviculture; Glyptostrobus pensilis (Lamb.) K. Koch; stem; tissue culture; organogenesis |
| 发表期数： | 2009年8月第16期 |
| 引用格式： | 李博，李火根，王光萍. 利用水松实生苗茎段诱导植株再生[J]. 中国科技论文在线精品论文，2009，2（16）：1711-1716. |
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