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直接利用糖质原料生产L-丝氨酸菌株的选育
发表时间:2009-10-15 浏览量:2067 下载量:606
全部作者: | 徐国强,张晓梅,窦文芳,许泓瑜,许正宏 |
作者单位: | 江南大学医药学院制药工程研究室;江南大学工业生物技术教育部重点实验室 |
摘 要: | 以一株能直接利用糖质发酵生产L-丝氨酸(L-Serine, L-Ser)的谷氨酸棒杆菌(Corynebacterium glutamicum)SYPS-062 为出发菌,经硫酸二乙酯(diethyl sulfate, DES)逐级诱变处理,在D-丝氨酸(D-Ser) 抗性梯度平板上挑取正突变株,获得一株遗传性能稳定的突变株33a-18,可积累L-Ser 11.0 g/L,较出发菌株提高了65.4%� |
关 键 词: | 发酵工程;L-丝氨酸;诱变育种;谷氨酸棒杆菌 |
Title: | Study on the mutant breeding of Corynebacterium glutamicum SYPS-062, direct-producing L-Serine from sugar |
Author: | XU Guoqiang, ZHANG Xiaomei, DOU Wenfang, XU Hongyu, XU Zhenghong |
Organization: | Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University; |
Abstract: | Corynebacterium glutamicum SYPS-062, which could use sugar to produce L-Ser, was used as an original strain for stepwise mutagenic treatment by diethyl sulfate (DES). Then positive mutants were selected on D-Ser resistance gradient plate. Finally a strain named 33a-18 was obtained. The 33a-18 can accumulate 11.0 g/L of L-Ser, which is increased by 65.4% compared with the original strain. |
Key words: | fermentation engineering; L-Serine; mutagenesis; Corynebacterium glutamicum |
发表期数: | 2009年10月第19期 |
引用格式: | 徐国强,张晓梅,窦文芳,等. 直接利用糖质原料生产L-丝氨酸菌株的选育[J]. 中国科技论文在线精品论文,2009,2(19):1994-1998. |

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